摘要
目的:观察益气化瘀方对腰神经根压迫损伤后神经根组织和比目鱼肌中脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)表达的影响。方法:SD雄性大鼠120只随机分为假手术组、模型组、弥可保(甲钴胺)组和益气化瘀方组,每组各30只。采用自制硅胶片压迫于大鼠腰椎(L4)右侧神经根背根节处建立损伤模型,分别给予生理盐水灌胃、弥可保注射液和益气化瘀方治疗。分别于治疗10、30和60d后处死大鼠,每组10只,取右侧受压神经根及比目鱼肌。采用透射电子显微镜观察受压神经根超微结构,酶联免疫吸附测定法检测压迫后神经根中BDNF蛋白表达,实时定量逆转录聚合酶链反应法检测比目鱼肌中BDNF mRNA的表达。结果:压迫损伤后各时间点模型组神经根中BDNF蛋白表达水平变化轻微,与假手术组相比,差异无统计学意义;益气化瘀方组BDNF含量在损伤后逐渐升高,60d时达到最高值,与模型组相比差异有统计学意义(P<0.01)。与假手术组比较,模型组比目鱼肌中BDNF mRNA的表达在损伤后10d时显著升高(P<0.01),随后逐渐降低,至60d时降至正常水平(P>0.05);与模型组相比,损伤后10d时益气化瘀方组比目鱼肌中BDNF mRNA表达升高受到显著抑制,60d时表达显著升高(P<0.01)。结论:益气化瘀方可以抑制神经根损伤早期靶器官中BDNF蛋白的升高,并可以促进神经损伤后期神经根及靶器官中BDNF的表达。
Objective： To observe the effects of Yiqi Huayu Decoction, a compound traditional Chinese herbal medicine, on brain-derived neurotrophic factor （BDNF） expression in lumbar nerve root and soleus of rats after lumbar nerve root compression injury.Methods： A total of 120 male SD rats were randomly allocated into sham-operated group, untreated group, methycobal injection group and Yiqi Huayu Decoction group. Surgery were performed on rats of untreated group, methycobal injection group and Yiqi Huayu Decoction group by placing a micro-silica gel on right L, dorsal root ganglia, while rats in sham-operated group received skin and paravertebral muscle incision only. Rats in the methycobal injection group and the Yiqi Huayu Decoction group were given intramuscular injection of methycobal and intragastric administration of Yiqi Huayu Decoction respectively. Rats in the sham-operated group and the untreated group received intragastric administration of normal saline of equal volume as Yiqi Huayu Decoction. The compressed nerve roots and right soleus were harvested after 10-, 30- and 60-day treatment. Microstructure of the compressed nerve roots were revealed under a transmission electron microscope. BDNF protein expression in the compressed nerve roots and mRNA expression in right solues were detected with enzyme-linked immunosorbent assay （ELISA） and real-time quantitative reverse transcription-polymerase chain reaction respectively. Results： The protein expression level of BDNF in compressed nerve root in the untreated group was slightly changed with no significance as compared with sham-operated group. BDNF protein expression in the Yiqi Huayu Decoction group was gradually increased and reached the peak level after 60-day treatment with significant difference as compared with the untreated group （P〈0. 01）. The expression of BDNF mRNA in soleus was significantly up-regulated 10 days after nerve root injury （P〈0. 01）, and was slowly decreased to the normal level at day 60 （P〈0. 05）. Compared with the untreated group, BDNF mRNA expression in soleus muscle in the YiqJ Huayu Decoction group was significantly down-regulated after 10-day treatment （P〈0.01）, while was increased after 60-day treatment （P〈0. 01）. Conclusion： Yiqi Huayu Decoction can inhibit the increase of BDNF expression at the early phase after nerve injury, which may have something to do with alleviation of neuropathic pain, and increase BDNF expression at the late phase to promote nerve regeneration.
出处
《中西医结合学报》
CAS
2010年第3期280-286,共7页
Journal of Chinese Integrative Medicine
基金
上海市教育委员会科研创新资助项目(No.08YZ56)
上海市科技启明星资助项目(No.07QA14051)
国家杰出青年科学基金资助项目(No.30625043)
国家自然科学基金青年基金资助项目(No.30600829
No.30701118
No.30801478)
上海市教育委员会博士点基金资助项目(No.2006B10)
上海市优秀学科带头人计划资助项目(No.08XD1404000)
关键词
神经根损伤
复方
脑源性神经营养因子
大鼠
nerve root injury
compound （TCD）
brain-derived neurotrophic factor
rats
