摘要
背景:研究表明壳寡糖具有抗肿瘤作用,但壳寡糖对cyclin D1、bcl-2和bcl-xl影响机制尚不清楚。目的:观察壳寡糖抑制Hela细胞生长的作用,以及对细胞内cyclin D1、bcl-xl和bcl-2 mRNA表达的影响。方法:壳寡糖(0.1,0.5,1,2,5g/L)刺激Hela细胞后,CCK8实验检测其对细胞生长的影响;运用实时荧光定量PCR方法在分子水平检测cyclin D1、bcl-xl和bcl-2 mRNA在细胞中的表达情况。结果与结论:壳寡糖可抑制Hela细胞的生长;随着壳寡糖的质量浓度由0.1g/L增加至2g/L,壳寡糖抑制cyclin D1,bcl-2,bcl-xl基因表达的作用也逐渐增强,在2g/L时作用最强,但质量浓度过高时(5g/L)抑制作用反而有所减弱。结果表明壳寡糖的抗肿瘤作用可能通过两方面进行:一方面壳寡糖可以抑制与细胞周期有关的cyclin D1 mRNA表达以抑制肿瘤细胞的增殖;另一方面壳寡糖可以抑制抗凋亡蛋白Bcl-2和bcl-xl mRNA的表达以促进肿瘤细胞的凋亡;且对前者的作用强于后者。
BACKGROUND：Studies have shown that chitooligosaccharides have antitumor effect.However,the influence of chitooligosaccharides on cyclin D1,bcl-2 and bcl-xl remains unclear.OBJECTIVE：To observe the inhibition effect of chitooligosaccharides on the proliferation of Hela cells,and the influence on cyclin D1,bcl-xl and bcl-2 mRNA expression.METHODS：Hela cells were stimulated by different concentrations of chitooligosaccharides（0.1,0.5,1,2,5 g/L）.The effects of chitooligosaccharides on Hela cells were detected by CCK8 kit.Using real-time PCR methods,the gene expression of cyclin D1,bcl-xl and bcl-2 mRNA were determined.RESULTS AND CONCLUSION：Chitooligosaccharides inhibited the proliferation of Hela cells.With the concentrations of chitooligosaccharides increased from 0.1 g/L to 2g/L,the inhibition effects on the gene expression of cyclin D1,bcl-xl and bcl-2 were enhanced,peaked at 2 g/L,and decreased at high concentration（5g/L）.Antitumor activity of chitooligosaccharides may exert through two aspects：it inhibits cyclin D1 mRNA expression to suppress the proliferation of tumor cells;on the other hand,chitooligosaccharides inhibits the expression of bcl-xl and bcl-2 to promote the apoptosis of tumor cells.Moreover,the effects of the former are stronger than the latter.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第3期429-432,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家高新技术发展计划(863计划)
编号:2007AA091603课题名称:功能性壳聚糖基组织器官修复材料的产业化技术研究~~
