摘要
目的观察包裹载多柔比星微球骨水泥的抗肿瘤活性。方法将细胞密度为1×106个/ml的SaoS-2细胞悬液,接种培养5d。用CCK-8试剂在免疫酶标仪上测定其吸光度值。用生理盐水调整细胞数为2×107/ml,取0.2ml肿瘤细胞于每只裸鼠左臀部消毒后皮下接种。从生理盐水调整的细胞数为2×107/ml的细胞悬液中取0.1ml,再分别加入3种已配制好的骨水泥浸提液0.1ml,分别按高、中、低剂量注射于每只裸鼠右耳后、左耳后及右臀部。每天观察裸鼠的活动、注射部位的皮肤和肿瘤生长情况。于实验第12天称瘤体质量并计算抑瘤率,常规HE染色观察。结果不同剂量组的包裹多柔比星微球骨水泥浸提液有一定抑制肿瘤生长的作用。各组浸提液对细胞增殖的抑制作用存在显著差异,以高剂量组最强,低剂量组最弱。各浸提液组均有明显的抑瘤作用。高剂量组抑瘤率达61.0%(P<0.05),剂量越小,抑瘤率也越小。HE染色后,大剂量浸提液组中可出现广泛的肿瘤坏死,肿瘤数量明显较少,而小剂量的浸提液抑瘤作用不明显,和模型组的肿瘤组织差异不明显。结论包裹多柔比星骨水泥的浸提液在体外和体内都具有良好的抗肿瘤活性。
Objective To investigate the antitumor effect of calcium phosphate cement incorporated with doxorubicin microspheres.Methods The absorbance at 490 nm of SaoS-2 cells cultured for 5 days in the media containing the extract of the cement incorporating doxorubicin microspheres was measured using Cell Counting Kit-8.SaoS-2 cells were adjusted to the density of 2×10^7 ml^-1 and injected into the left buttock of nude mouse in the volume of 0.2 ml.The cell suspension（0.1 ml） mixed with an equal volume of the cement extract were injected into the right buttock and on the back of the bilateral ears of nude mice.At 12 days after the cell injection,the tumor tissues were obtained and weighed to calculate the tumor inhibition rate,and the pathological samples were observed with HE staining.Results The extract of the bone cement containing doxorubicin microspheres showed inhibitory effects on the tumor growth in a dose-dependent manner.The tumor inhibition rate reached 61.0% in high-dose group.Tumor necrosis was found in high dose group,but virtually absent in low-dose group.Conclusion CPC containing doxorubicin PLGA microspheres can inhibit tumor cell growth both in vitro and in vivo.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2010年第2期278-283,共6页
Journal of Southern Medical University
基金
广东省科技计划项目(2007B031003009)
关键词
磷酸钙骨水泥
微球
多柔比星
抗肿瘤活性
calcium phosphate cement
microspheres
doxorubicin
tumor inhibition
