摘要
目的：探索静态瞬稳注射化学发光法检测大豆异黄酮方法。方法：本实验在氢氧化钠-铁氰化钾体系下对大豆异黄酮进行化学发光分析,确定较佳实验条件,利用光强度测定大豆异黄酮含量。结果：获得理想发光曲线的实验条件是在-1000 V负高压、30 s发光时间,当铁氰化钾溶液浓度为0.16 mol/L,氢氧化钠溶液浓度为0.04 mol/L时,整个反应体系的化学发光最强。大豆异黄酮的浓度在0～200.00 mg/L范围内,与化学发光强度成良好线性关系,相关系数r=0.997。结论：此方法简单,周期短,成本低。加标回收率95%～110%,RSD5%（n=3）,检测限为0.1 mg/L,测试结果较理想,能满足大豆异黄酮产品质量检测的需要。
Objective：To explore a new method for determiniation of soybean isoflavones by static instantaneous steady injection chemiluminescence.Methods：In this experiment,soybean isoflavones was detected and analyzed by using chemical luminescence with high sensitivity in the sodium hydroxide-potassium ferricyanide system,and the best experimental conditions was found out.Results：The luminescence curve of the most idea experimental conditions was negative high voltage-1000 V,light-emitting time of 30 s.And the concentration of potassium ferricyanide solution was 0.16 mol/L,sodium hydroxide was 0.04 mol/L.Chemiluminescence of the entire reaction system was the strongest.We could gain a good linearity with correlation coefficient 0.997 in the concentration of soybean isoflavones 0~200.00 mg/L.Conclusion：The method is simple,short-time and low-cost.The recoveries of standard addition is 95%~110%,and relative standard deviation lower than 5%（n=3）,detection limit 0.1 mg/L.The test result is ideal.This new method should provide experimental basis and quality inspection needs for the detection of soybean isoflavones.
出处
《中国卫生检验杂志》
CAS
2010年第3期538-540,共3页
Chinese Journal of Health Laboratory Technology
