摘要
目的:观察白细胞介素6(IL-6)对人单核/巨噬细胞系(THP-1)细胞表面Toll样受体4(TLR4)表达的影响。方法:流式细胞仪检测不同浓度IL-6(0ng/L、10ng/L、100ng/L、1μg/L、10μg/L、100μg/L)干预THP-1细胞24h,以及10μg/L的IL-6干预THP-1细胞不同的时间(0、3、6、12、24、48h)后细胞表面TLR4的表达水平。结果:各浓度IL-6均能不同程度的促进细胞表面TLR4的表达(P<0.05),以10μg/L组干预最为显著(P<0.01);10μg/LIL-6干预不同的时间后均可促进其表达,以干预24h后的差异最显著(P<0.01)。结论:IL-6可以促进人THP-1细胞表面TLR4表达,且具有一定的浓度和时间依赖性。
Objective：To explore the effects of IL-6 on the expression of Toll like receptor-4（TLR4） of THP-1. Methods：Flow Cytometry （FCM） was used to detect the expression of TLR4 of THP-1 cells,which was stimulated with different concertrations of IL-6 （0 ng / L,10 ng / L,100 ng / L,1 μg / L,10 μg / L and 100 μg / L）,and on different time after treated with 10 μg / L IL-6 （0 h,3 h,6 h, 12 h,24 h,48 h）. Results：Different concertrations of IL-6 can significantly promote the expression of TLR4 in THP-1 cells,especially under the concentration of 10 μg/L IL-6 group. Still this effect can be observed in 10 μg/L IL-6 group under treated for 3 h, 6 h,12 h, 24 h and 48 h,especially at 24 h. Conclusion：The expression of of TLR4 on THP-1 cells can be promoted by IL-6 in a dose-and timedependence.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第3期319-323,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省卫生厅创新基金(XK12200903)
