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雷公藤甲素对TNF-α诱导的滑膜细胞增殖的影响及对Ras-MAPKs信号转导通路的调控作用 被引量:29

Effects of triptolide on cell proliferation and regulation of Ras-MAPKs pathway in synoviocytes induced by tumor necrosis factor
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摘要 目的:探讨雷公藤甲素(TP)对肿瘤坏死因子(TNF-α)诱导的类风湿关节炎患者成纤维样滑膜细胞(RA-HFLS)增殖的影响及对Ras-MAPKs信号转导通路的调控作用。方法:不同浓度的TP(0.28,2.8,28,140 nmol.L-1)与RA-HFLS共孵育,采用MTS比色法检测细胞增殖活性,并通过Western blot检测不同浓度的TP对TNF-α诱导的RA-HFLS Ras-MAPKs信号转导通路相关蛋白(Ras,p-P38,p-ERK,p-JNK)表达水平的影响。结果:MTS结果显示,TP可以剂量依赖性抑制RA-HFLS的增殖,其抑制率分别为0.28%,5.05%,30.83%,43.77%;Western blot结果提示TP可剂量依赖性抑制Ras-MAPKs信号转导通路相关蛋白的表达。结论:TP能抑制RA-HFLS增殖、减轻滑膜炎症,其分子机制可能与调控了Ras-MAPKs信号转导通路的异常活化有关。 Objective: To investigate the effects of triptolide on proliferation and regulation of Ras-MAPKs pathway in human fibroblast-like synoviocytes of Rheumatoid Arthritis(RA-HFLS) treated with tumor necrosis factor (TNF-α). Methods: RA-HFLS were cultured with TNF-α in the presence or absence of variable doses of triptolide (0.28, 2.8, 28, 140 nmol?L-1) in vitro. Cell proliferation was evaluated by MTS assay. The phosphorylation status of Ras-MAPKs-associated proteins (Ras,p-P38,p-ERK and p-JNK) were detected by Western blot analysis. Results: Triptolide could obviously decrease the RA-HFLS viability in a dose-dependent style and the inhibition ratio were 0.28%, 5.05%, 30.83% and 43.77% respectively. In addition, triptolide could also suppressed the expression of Ras, p-P38, p-ERK and p-JNK. Conclusion: Triptolide has an notable inhibiting effect on proliferation of RA-HFLS and the molecule mechanism is due in part to the direct suppression of abnormal activation of Ras-MAPKs pathway.
出处 《中国中药杂志》 CAS CSCD 北大核心 2010年第7期888-891,共4页 China Journal of Chinese Materia Medica
基金 北京市自然科学基金项目(7062051)
关键词 类风湿关节炎 成纤维样滑膜细胞 雷公藤甲素 MAPKS Rheumatoid arthritis human fibroblast-like synoviocytes of Rheumatoid Arthritis triptolide MAPKs
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参考文献12

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