摘要
目的通过检测炎症性肠病(IBD)患者血清胰岛素样生长因子(IGF-1)水平以及肠道黏膜组织中IGF-1的表达,探讨IGF-1与IBD活动度和病变部位之间关系。方法收集2008年1月—10月诊断为IBD的患者75例,其中克罗恩病(CD)30例,溃疡性结肠炎(UC)45例,收集同期行结肠镜检查未见异常且年龄、性别构成与IBD组相近的39例患者作为对照组。根据Harvey和Bradshow标准的疾病活动指数(简化CDAI)及Southerland疾病活动指数(UCAI)对CD和UC患者的疾病程度进行分级。测定所有患者的血清IGF-1水平以及肠道黏膜组织的IGF-1表达。结果活动期UC和CD患者的血清IGF-1水平分别为(149.37±101.81)、(112.23±45.94)ng/mL;缓解期分别为(159.4±91.63)、(142.89±121.61)ng/mL,均显著低于对照组的(236.00±62.55)ng/mL(P值均<0.05)。但活动期与缓解期亚组间的差异均无统计学意义(P值均>0.05)。UC组中,全结肠型和部分结肠型患者的血清IGF-1水平分别为(157.56±96.60)、(140.47±101.68)ng/mL,两亚组间的差异无统计学意义(P>0.05)。CD组中,结肠累及型和小肠型患者的血清IGF-1水平分别为(97.48±29.35)、(145.90±113.14)ng/mL,两亚组间的差异无统计学意义(P>0.05)。IBD组患者的肠道黏膜IGF-1呈高表达,而对照组呈低表达。结论血清IGF-1对判断IBD的活动度、病变部位的特异性不强。IBD患者的肠壁组织中可见IGF-1蛋白表达显著增强,可能肠间质细胞局部分泌的IGF-1介导了炎症的发生、发展。
Objective To explore the serum level of insulin-like growth factor-1 （IGF-1） and IGF-1 expression in intestinal mucosa of patients with inflammatory bowel disease （IBD）, and to investigate the relationship between IGF-1 and the activity and involvement area of IBD. Methods Seventy-five IBD patients, 45 with ulcerous colitis （UC） and 30 with Crohn disease （CD）, who were treated during Jan. 2008 to Dec. 2008, were included in the present study. The patients were classified according to Bradshow Classification （CDAI） and Southerland Activity Index （UCAI）. Meanwhile, 39 age- and sex-matched controls were also included in this study. The serum levels of IGF-1 and the expression of IGF-1 in intestinal mucosa were determined. Results The serum levels of IGF-1 in patients with active UC（rep） and CD （rep） were （ 149.37±101.81 ） ng/mL and （ 112.23 ±45.94） ng/mL, respectively; and those in patients at remission stage of UC（rep） and CD （rep） were （159.4± 91.63） ng/mL and （142.89±121.61） ng/mL, respectively; all the 4 numbers were significantly lower that in the controls （[236.00±62.55] ng/mL, P〈0.05）. But there were no statistical differences between the active and remission stages in the UC and CD groups （P〉0.05）. The levels of IGF-1 in full colon involvement and segmental colon involvement patients with UC were not significantly different （[ 157.56 ± 96.60） ng/mL vs. [140.47± 101.68） ng/mL, P〉 0. 05） . The levels of IGF-1 in colonic CD patients and enteric CD patients were not significantly different （[97.48 ±29. 351 ng/mL vs. [145.90 ±113. 141 ng/mL, P〉0.05）. IGF-1 was highly expressed in the mucosa of IBD patients and lowly expressed in the controls （P〈0.05）. Conclusion Serum IGF-1 level may not specifically demonstrate the activity and involvement of IBD. IGF-1 protein is highly expressed in the mucosa of IBD patients, indicating that IGF-1 secreted by mesenchymal cells may mediate the development of fibrosis in IBD patients.
出处
《上海医学》
CAS
CSCD
北大核心
2010年第1期104-106,F0002,共4页
Shanghai Medical Journal
关键词
炎症性肠病
溃疡性结肠炎
克罗恩病
胰岛素样生长因子
Inflammatory bowel disease
Ulcerative Oolitis
Crohn＇s Disease, Insulin-like growth factor-1
