摘要
目的：了解有机磷和拟除虫菊酯两类杀虫剂对家蝇线粒体膜脂流动性的影响。方法：采用荧光偏振法，以ＤＰＨ为荧光探剂。结果：６种有机磷化合物和４种拟除虫菊酯与家蝇线粒体膜作用３～６分钟，即可引起膜流动性的明显改变。对膜流动性影响的方式因杀虫剂结构上的差异而有所不同。氯菊酯和倍硫磷、马拉硫磷、敌百虫、氧化乐果可使流动性增加，而氰戊菊酯、氯氰菊酯、溴氰菊酯和辛硫磷、乙酰甲胺磷则可使膜流动性下降。不同杀虫剂对相变温度的影响也存在差异。溴氰菊酯和乙酰甲胺磷、氧化乐果、倍硫磷可使相变温度降低１℃，而氯氰菊酯和马拉硫磷、敌百虫则分别使相变温度增加２、３和３℃，但氯菊酯对相变温度的影响不明显。结论：这两类杀虫剂对生物膜流动性的影响。
This paper deals with the effects of four pyrethroids and six organophosphates on the fluidity and phase transition temperature of the mitochondrial membrane with fluorescence polarization technique using DPH as a probe. The results showed that the influence of the kind of pyrethroids and organophosphates on membrane fluidity were different. The presence of permethrin or Fenthion、Malathion、Trichlorfon and Omethoate increased the fluidity. But the membrane fluidity were decreased by adding Fenvalerate、Cypermethrin、deltamethrin or phoxim and acephate. Further effect of pyrethroids and organophosphates on the mitochondrial membrane was the shift of the phase transition temperatures. The phase transition temperature decreased by 1℃ in the prescence of Deltamethrin or Acephate、 Omethoate、Fenthion and increased by 2℃、3℃ and 3℃ respectively when adding Cypermethrin or Malathion、 Trichlorfon. Permethrin has no significant effect to phase transition temperature. The results suggest that the effect of pyrethroids and organophosphates on the membrane fluidity may be a critical element in their action mechanisms.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
1998年第6期429-435,共7页
Chinese Journal of Vector Biology and Control
关键词
杀虫剂
家蝇
飞翔肌线粒体
荧光偏振
膜流动性
Insecticides Housefly Flight muscle mitochondrial Fluorescence polarization Membrane fluidity
