摘要
本文以正十九碳酸（Ｃ１９：０）作内标，用ＨＣＩ－ＣＨ３ＯＨ对海洋微藻（三角褐指藻）进行抽提酯化后做毛细管气相色谱分析。方法的重现性各脂肪酸的相对偏差为０．３％～１１．６％，回收率为８５．７％～１０３．３％，仪器稳定性的相对偏差为０．２％～３．１％。
A capillary column gas chromatographic technique for the determination of microalgae ( phaeodactylum tricornutum )fatty acids has been developed.The fatty acid extraction and esterification is finished in one step.Eleven kinds of fatty acid methyl esters have been identified.The CV is less than 7% except C 22:5ω3 and the recovery rate 85.7～103.3%.Thus a rapid,convenient,reliable and microalytical method is established for the study on developing and making use of microalgae.
出处
《海洋技术》
1998年第4期64-68,共5页
Ocean Technology
