摘要
目的观察链脲佐菌素(STZ)诱导糖尿病大鼠心肌组织超微结构的变化及抵当芪桂汤对其超微结构的影响。方法健康雌性SD大鼠50只,随机抽取10只为空白组,其余40只大鼠以STZ诱导成糖尿病模型。造模成功后随机分为模型组、达美康组、抵当芪桂汤组,给予相应干预。8周后检测大鼠的血糖、血脂等生化指标,用H-600透射电镜和普通光镜观察STZ大鼠心肌组织结构的变化。结果(1)造模成功后,模型组大鼠血糖明显升高(P<0.01)。(2)干预结束后,与模型组比较,达美康组、抵当芪桂汤组大鼠心肌细胞肥大及坏死有所改善,细胞间质减少,肌丝排列相对整齐,抵当芪桂汤组改善较明显。结论糖尿病大鼠心肌超微结构损伤明显,抵当芪桂汤可以改善STZ诱导糖尿病大鼠心肌超微结构,从而延缓糖尿病心肌病变的发生和发展。
Objective To investigate the change of myocardial uhrastructure in streptozotocin （STZ） - induced diabetic rats, and the protective effects of Didangqigui Recipe on myocardial ultrastructure. Methods Ten of 50 healthy male SD rats were chosen randomly as blank group, the other 40 were STZ - induced into diabetic models, which were randomized into groups model, gliclazide, Didangqigui after establishment, receiving corresponding intervention. The blood glucose, lipid and other biochemical indicators were detected, and the changes of myocardial tissue structure of STZ rats were observed by H - 600 transmission electron microscope and ordinary light microscope. Results STZ rats had higher blood glucose after model establishment （P 〈 0. 01 ）. The cardiac hypertrophy and necrosis improved, interstitial cells reduced, myofilament arrangement was relatively neat in groups gliclazide and Didangqigui as compared with model group after intervention, but Didangqigui group improved more. Conclusion Myocardial uhrastructure injury is obvious in diabetic rats. Didangqigui recipe can improve the myo- cardial ultrastructure of STZ - induced diabetic rats, thus to delay the occurrence and development of diabetic cardiomyopathy.
出处
《中国全科医学》
CAS
CSCD
北大核心
2010年第11期1201-1203,共3页
Chinese General Practice
基金
陕西省自然科学基础研究计划项目(2007C242)
陕西省教育厅科研计划项目(08JK271)
