摘要
Ａ组轮状病毒是导致婴幼儿重症腹泻的最主要病毒病原，ＶＰ７是病毒外壳上的主要糖蛋白，它具有中和抗原活性，与病毒的毒力及免疫保护性有关，也是划分病毒血清型的最主要标志之一〔１〕。ＶＰ７基因及其编码蛋白一直是人们研究的主要对象，很多研究工作和诊断试剂都需大...
The full length of G2 type VP7 cDNA was amplified by reverse transcription and polymerase chain reaction(RT-PCR)from a group A rotavirus field strain CR100 circulating in Beijing. The amplified cDNA was directly cloned into the transfer vector pBacPAK8 downstream of the polyhedrin promoter. Sequence analysis showed 95.7% homology of the deduced amino acid between VP7s of CR100 and prototype strain Hu-5. Insect cells were cotransfected with recombinant plasmid pBacPAK8-CR100 and Bsu36I-digested BacPAK6 viral DNA. Recombinant baculovirus containing CR117 VP7 gene was obtained by plaque screening and PCR identification using the forward primer on the polyhedrin promoter and the downstream primer of VP7. Specific VP7 with three different molecular weights was detected by Western blot using hyperimmune serum against rotavirus strain DS-1(G2 type)when expressed in insect cells.
出处
《病毒学报》
CAS
CSCD
北大核心
1998年第4期374-376,共3页
Chinese Journal of Virology
基金
国家自然科学基金
卫生部科研基金
北京市自然科学基金
