摘要
利用ＲＡＰＤ分析技术，选取２２个２０个碱基长度的随机引物，对７种野生菊花、１４种栽培菊花和５个种间杂种进行了随机扩增。通过实验建立了ＰＣＲ随机扩增实验体系。在观察到的２２４个扩增条带中，３４条（１５％）表现多态性。利用ＵＰＧＭＡ法对扩增数据进行分析，结果表明：在７种野生种中，Ｄｅｎｄｒａｎｔｈｅｍａｉｎｄｉｃｕｍ、Ｄ．ｖｅｓｔｉｔｕｍ和Ｄ．ｎａｎｋｉｎｇｅｎｓｅ与栽培菊花亲缘关系最近，而Ｄ．ｚａｗａｄｓｋｉ与栽培菊花亲缘关系最远。前３种野菊与栽培菊花间的遗传距离小于０．４０，而Ｄ．ｚａｗａｄｓｋｉ与栽培菊花间的遗传距离大于０．５０。根据上述数据及以往研究结果，使用ＲＡＰＤ数据对菊花起源问题进行了探讨，提出栽培菊花主要起源于Ｄ．ｉｎｄｉｃｕｍ、Ｄ．ｖｅｓｔｉｔｕｍ和Ｄ．ｎａｎｋｉｎｇｅｎｓｅ．
The RAPD ( random amplified polymorphic DNA) markers generated by 22 20 mer primers, discriminated 7 wild Dendranthema species, 14 cultivars and 5 interspecific hybrids by banding profiles. Of 224 bands observed, 34 bands (15%) were polymorphic. Cluster analysis based on UPGMA method indicated that: of 7 wild species, D. indicum, D. vestitum and D. nankingense were closest to the cultivated groups while D. zawadskii was most distinct. Genetic distances between the former three species and cultivars were less than 0.40 but those between D. zawadskii and cultivars were more than 0.50. According to the RAPD analysis and previous research, the authors discussed the origin of Chinese cultivated chrysanthemum and proposed that the cultivars of this genus may derive from D. indicum, D. vestitum and D. nankingense. The results have provided basic research data for study on the origin of Chinese cultivated chrysanthemum.
基金
国家自然科学基金
