摘要
将细胞的显微分离、微量蛋白电泳和超敏感银染有机结合起来，对显微分离的大蒜根端分生组织间期、前期、中期、后期和末期各１００个细胞进行了蛋白电泳。发现在不同时期，含量较高的蛋白谱带有７条，其中４５ｋＤ和６５ｋＤ的蛋白在间期、前期、中期。
root tip cells at interphase, prophase, metaphase, anaphase and telophase of mitosis of Allium sativum L. have been isolated respectively and the proteins have been analyzed by combining micromanipulation with micro SDS PAGE and ultrasensitive silver staining. Comparing the protein change during the different phases it was shown that different patterns of cyclic changes occurred in various proteins. Some proteins changed with regularity which could be repeated.
基金
国家教委"跨世纪优秀人才计划"基金
关键词
显微分离
大蒜
有丝分裂
蛋白质
电泳
Micro isolation, Ultrasensitive silver staining, Micro SDS PAGE
