摘要
采用高效液相色谱法，利用C18柱（4．6mm×150mm，5μm），以乙腈：水（15：85）为流动相，检测波长277nm，建立中药复方“LH”中连翘苷含量的测定方法．结果表明，连翘苷的线性范围为0．05～2．5μg；相关系数r=0．9993，连翘苷平均回收率为96．83％，RSD=1.73％（n=6）．该测定法灵敏，简便，重现性好，结果准确，可用于中药复方“LH”中连翘苷的含量测定．
The separation was carried out on C,.8 （4.6 mm× 150 mm,5 μm）column, the mobile phase was composed of aeetonitrile to H2O（15：85）, detection wavelength was 277 nm. The results showed that the linear range of paeoniflorin is 0.05-2.5 μg （r=0. 999 9）, average recovery is 96.83% （RSD=1. 73%, n=6）. The method is sensitive, rapid, convenient, reproducible and accurate, and suitable for the content determination of ＂LH＂
出处
《延边大学农学学报》
2010年第1期62-64,共3页
Agricultural Science Journal of Yanbian University
关键词
HPLC
中药复方“LH”
连翘苷
HPLC
Chinese traditional compound medicine ＂LH＂forsythin
