摘要
目的建立健康成人血液T淋巴细胞亚群的参考区间。方法使用三标荧光抗体,分别对100例不同年龄性别健康成年人外周血T淋巴细胞亚群进行标记,采用EPICSXL/XL-MCL型流式细胞仪进行检测并分析结果,SPSS13.0软件进行统计学处理。结果18～65岁健康成年人CD3+、CD3+/CD4+、CD3+/CD8+、CD4+/CD8+正常95%范围分别为59.17～76.15、24.26～40.50、18.76～27.40、0.62～2.42;不同年龄段比较中51～65岁CD3+、CD3+/CD4+、CD3+/CD8+、CD4+/CD8+各项指标与18～30岁比较差异有统计学意义(P<0.05)。结论在相同地域、相同种族,可以建立健康成年人外周血的T淋巴细胞亚群正常参考区间。
Objective To investigate the level of peripheral blood T lymphocyte subsets in the adults of different ages and sexes in Zhanjiang area and to establish the reference range for normal values of T lymphocyte subsets in healthy adults in Zhanjiang area. Methods Peripheral blood T lymphocyte subsets of 100 health adults in different ages and sexes were marked by three-color fluorescent labeling, and the results were detected and analyzed by Beck man Coulter Epics XL flow cytometry. The statistical analysis of the results were conducted by SPSS 13.0 software. Results The range of 95% normal values of CD3 ^＋ , CD3^＋/CD4 ^＋ , CD3^＋/CD8^＋ , CD4^＋nd ＋/CD8 in healthy adults aged from 18 to 65 are 67.66±8.49,32.48±8.02,28.08±9.32,1.52 and ±0.90 respectively. Compared to the CD3^＋,CD^＋/CD4^＋ ,CD3^＋/CD8^＋ ,CD4^and ＋/CD8 in the groups with different ages between 51 - 65 and 18- 30 years old, the P values of the statistical significance are 0. 007,0. 032,0. 040, and 0. 046 respectively. Conclusion The reference range for normal values of T lymphocyte subsets in healthy adults in the same area and the same race can be established.
出处
《检验医学与临床》
CAS
2010年第7期596-597,共2页
Laboratory Medicine and Clinic
关键词
T淋巴细胞亚群
正常参考区间
流式细胞仪
category of T lymphocyte subsets
range of normal value
flow cytometry
