摘要
目的:观察健足颗粒(加减防己黄芪汤)对糖尿病皮肤溃疡大鼠血管内皮功能的影响。方法:选用SD雄性大鼠90只,随机选取6只为正常对照组,其余大鼠以尾静脉注射四氧嘧啶40mg/kg,筛取符合糖尿病诊断标准的大鼠用于建立糖尿病皮肤溃疡模型,并随机分成6组,模型对照组、健足颗粒干预的高、中、低剂量组,中药对照组、西药对照组。实验结束后断头取血检测NO、ET-1含量以观察健足颗粒对血管内皮功能的影响。结果:与模型组比较,各治疗组大鼠血清NO含量均有不同程度升高,而血浆ET-1含量却有不同程度的降低,其中以健足颗粒高剂量组变化最明显(P<0.001),其次为中剂量(P<0.01),低剂量、中药对照组次之(P<0.05),西药对照组也有轻度改变,但无显著统计学意义(P>0.05)。健足颗粒各剂量组的调节作用与剂量呈现相关性。结论:健足颗粒可通过升高血清NO含量、降低血浆ET-1水平来改善血管内皮功能,有效防治糖尿病皮肤溃疡发生发展。
Objective：To observe the effects of JIANZU Granule （Modified Fangji Huangqi Decoction） on vascular en- dothelial function of Rats with Diabetic Skin Ulceration （DSU）. Methods ： 90 SD rats in male were randomized into two groups, namely Normal Control Group （NCG） with 6 rats, Model Group（MG） of 84 rats with tail vein injection of allox- an 40mg/Kg. Selecting the rats meeting the diagnostic criteria of Diabetes to establish Diabetic skin ulcer model for fur- ther observation, then randomly divided into 6 groups, Model Control Group（ MCG）, JIANZU Granule （JZG） High -dose Group, Middle -dose Group and Low - dose Group, Tang Maikang Control Group （TMCG）., Mefformin plus 654 - 2 Control Group（MNCG）. In the end of the experiment, it was decapitated blood test NO, ET - 1 to observe the effects of JZG on vascular endothelial function. Results： Compared with IVICG, serum NO levels were increased to some degrees in all treatment groups, but the plasma ET - 1 levels have different degrees of reduction. The most obvious change was observed in the High - dose Group （P 〈 0. 001 ）, significantly in Middle - dose Group （ P 〈 0.01 ）, followed by Low - dose Group and TMCG （ P 〈 0.05）. There was a slight change in MNCG, but no significant statistically significance （ P 〉 0.05 ）. The regulating funtion of each dose group was showed a dose - related. Conclusion ： JIANZU Granule has the function of impmvemeut of vascular endothelial function through increasing serum NO level, decreasing plasma level of ET - 1, ef- fectively preventing occurrence and development of Diabetic Skin Ulceration.
出处
《中华中医药学刊》
CAS
2010年第4期837-839,共3页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中药现代化专项资金资助项目(浙财建字[2005]241号)
关键词
糖尿病
皮肤溃疡
一氧化氮
内皮素-1
防治
健足颗粒
防己黄芪汤
diabetes mellitus
skin ulceration
NO
ET - 1
prevention and treatment
JIANZU Granule
Fangji Huan- gqi Decoction
