期刊文献+

肝纤维化与肝硬化患者肝内CXCL13和CXCL16的表达变化 被引量:3

Hepatic expressions of CXCL13 and CXCL16 in patients with hepatic fibrosis and cirrhosis and in normal controls
下载PDF
导出
摘要 目的观察肝纤维化与肝硬化患者肝组织中趋化因子CXCL13和CXCL16的表达变化。方法用ELISA方法分别检测9例正常人和10例肝纤维化、11例肝硬化患者肝组织中的CXCL13和CXCL16含量。结果正常对照组、肝纤维化组、肝硬化组的CXCL13浓度分别为(2.34±2.05)ng/g、(6.04±2.97)ng/g和(12.10±10.38)ng/g;肝硬化组的浓度显著高于正常对照组和肝纤维化组,正常对照组与肝纤维化组的CXCL13浓度差异无统计学意义。正常对照组、肝纤维化组、肝硬化组的CXCL16浓度分别为(1.32±0.91)ng/g、(3.34±1.81)ng/g和(3.49±1.90)ng/g;肝硬化组和肝纤维化组浓度均显著高于正常对照组浓度,肝硬化组和肝纤维化组的CXCL16浓度差异无统计学意义。结论肝脏发生纤维化损伤时,肝脏表达CXCL13和CXCL16的数量都显著增多,但两者的变化规律不同。肝内CXCL13水平在肝纤维化阶段开始升高,至肝硬化阶段升高到显著水平。肝内CXCL16水平在肝纤维化阶段就已经升高到显著水平,至肝硬化阶段仍维持在高水平状态。 Objective To investigate the expression of chemotactic factors (CXCL13 and CXCL16) in the liver of patients with hepatic fibrosis and cirrhosis and in normal controls. Methods Hepatic tissues were obtained in surgery from 9 normal persons,10 patients with hepatic fibrosis,and 11 patients with cirrhosis. Expression of CXCL13 and CXCL16 in hepatic tissues was quantified by ELISA. Results The concentrations of CXCL13 were 2.34±2.05,6.04±2.97 and 12.10±10.38 ng/g in normal,hepatic fibrosis and hepatic cirrhosis group,respectively. CXCL13 in sclerotic liver was higher than that in normal controls and fibrotic liver,but there was no significant difference. The concentrations of CXCL16 were 1.32±0.91,3.34±1.81 and 3.49±1.90 ng/g in normal,hepatic fibrosis and hepatic cirrhosis group. CXCL16 in fibrotic liver and sclerotic liver were higher than that in normal liver,but there was still no significant difference. Conclusion With the aggravation of hepatic fibrous degeneration,hepatic levels of CXCL13 and CXCL16 increase,but follow a different way. CXCL13 begins to rise in the stage of liver fibrosis and has a significant increase in cirrhosis stage. CXCL16 increases to significant level in the stage of liver fibrosis,and remains in a high state in liver cirrhosis stage.
出处 《广东医学》 CAS CSCD 北大核心 2010年第5期564-565,共2页 Guangdong Medical Journal
基金 广州市科技攻关计划项目(编号:2007Z3-E0371)
关键词 肝纤维化 肝硬化 CXCL13 CXCL16 趋化因子 fibrosis cirrhosis liver CXCL13/BCA-1 CXCL16 chemokine
  • 相关文献

参考文献9

  • 1LEGLER D F,LOETSCHER M,ROOS R S,et al.B cell-attracting chetnokine 1,a human CXC chemokine expressed in lymphoid tissues,selectively attracts B lymphocytes via BLR1/CXCR5[J].J Exp Med,1998,187(4):655-660.
  • 2徐焕宾,熊思东.新发现的CXCL16趋化因子及其受体[J].生命的化学,2003,23(1):8-10. 被引量:19
  • 3JUNG Y J,RYU K H,CHO S J,et al.Syngenic bone marrow cells restore hepatic function in carbon tetrachloride-induced mouse liver injury[J].Stem Cells Dev,2006,15(5):687-695.
  • 4廖彩仙,唐浩,周杰,苏俊,张守华,张春兴.小鼠骨髓源性肝干细胞筛选及其分化潜能的研究[J].中华肝胆外科杂志,2007,13(9):614-616. 被引量:5
  • 5HATCH H M, ZHENG D, JORGENSEN M L, et al. SDF-1alpha/CXCR4: a mechanism for hepatic oval cell activation and bone marrow stem cell recruitment to the injured liver of rats [ J ]. Cloning Stem Cells, 2002, 4(4) : 339 -351.
  • 6LISIGNOLI G, CRISTINO S, PlACENTINI A, et al. Hyaluronan - based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells : Involvement of Cd44 and Cd54[J]. J Cell Physiol, 2006, 207(2) : 364 -373.
  • 7STICH S, LOCH A, LEINHASE I, et al. Human periosteum -derived progenitor cells express distinct chemokine receptors and migrate upon stimulation with CCL2, CCL25, CXCL8, CXCL12, and CXCL13[J]. Eur J Cell Biol, 2008, 87(6): 365 -376.
  • 8SORDI V, MALOSIO M L, MARCHESI F, et al. Bone marrow mesenchymal stem cells express a restricted set of functionally active chemokine receptors capable of promoting migration to pancreatic islets[J]. Blood, 2005, 106(2) : 419 -427.
  • 9HONCZARENKO M, LE Y, SWIERKOWSKI M, et al. Human bone marrow stromal cells express a distinct set of biologically functional chemokine receptors[J ]. Stem Cells, 2006, 24(4) : 1030 - 1041.

二级参考文献17

  • 1刘水冰,田琼.小鼠性决定基因SRY探针的制备[J].华南国防医学杂志,2005,19(3):12-13. 被引量:7
  • 2唐浩,廖彩仙,周杰,金浩生,谭远飞,苏俊,张春兴,张守华.小鼠c-Kit^+lin-骨髓细胞移植生成肝细胞的实验研究[J].南方医科大学学报,2006,26(5):567-569. 被引量:23
  • 3[1]Matloubian M et al. Nat Immunol, 2000, 1(4): 298-304
  • 4[2]Wilbanks A et al. J Immunol, 2001, 166(8):5145-5154
  • 5[3]Kume N. Nippon Ronen Igakkai Zasshi, 2002, 39(3):264-267
  • 6[4]Bonecchi R et al. J Exp Med, 1998, 187:129-134
  • 7[5]Sharron M et al. Blood, 2000, 96(1):41-49
  • 8[6]Kim CH et al. J Clin Invest, 2001, 107(5): 595-601
  • 9[7]Jogodzinski PP et al. Folia Histochem Cytobiol, 2000, 38:21-23
  • 10[8]Brussel A et al. AIDS Res Hum Retroviruses, 1999, 15(14):1315-1319

共引文献22

同被引文献38

引证文献3

二级引证文献23

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部