摘要
建立了以超高效液相色谱-电喷雾串联四极杆质谱(UPLC-MS/MS)检测果汁和葡萄酒中27种工业染料的方法。样品经乙腈振荡提取,在盐析作用下分层,目标化合物转移至乙腈层中。目标化合物在梯度洗脱条件下经C18柱分离后采用多反应监测(MRM)模式进行检测。其中24种工业染料使用正离子模式检测,流动相为乙腈和0.1%的甲酸水溶液;其余3种工业染料则采用负离子模式检测,流动相为乙腈和水。结果表明:果汁中27种工业染料的定量限(LOQ)为0.1～50μg/kg,回收率为57.0%～117.7%,相对标准偏差为2.4%～17.7%。葡萄酒中的LOQ为0.2～50μg/kg,回收率为40.8%～109.4%,相对标准偏差为1.6%～17.9%。该方法操作简单,灵敏度高,实现了不同种类的工业染料的同时提取,适合于软饮料中非法添加工业染料的快速检测。
A method for the determination of 27 industrial dyes in juice and wine has been developed using ultra performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）.Acetonitrile was used as extraction solvent,and sodium chloride was added to salt out the analytes from the samples.Chromatographic separation was performed on a C18 column with the gradient elution and the mass spectrometric acquisition was carried out under the mode of multiple reaction monitoring （MRM）.Twenty-four of the 27 dyes were detected under positive ionization mode using the mobile phase of acetonitrile and water containing 0.1% formic acid.The other 3 dyes were analyzed under negative ionization mode with the mobile phase of acetonitrile and water.As a result,the average recoveries of 27 dyes spiked in juice ranged from 57.0% to 117.7% with the relative standard deviations （RSDs） of 2.4%-17.7%,and the average recoveries of 27 dyes spiked in wine ranged from 40.8% to 109.4% with the RSDs of 1.6%-17.9%.The limits of quantification （LOQs） of 27 dyes spiked in juice were in the range of 0.1-50 μg/kg,and 0.2-50 μg/kg for those spiked in wine.This method can be applied to rapid detection of illegally added dyes in soft drinks due to its simplicity and high sensitivity.
出处
《色谱》
CAS
CSCD
北大核心
2010年第4期356-362,共7页
Chinese Journal of Chromatography
基金
北京市科技计划项目(No.D08050200310802)
卫生部公益性行业科研专项项目(No.200902009-02)
关键词
超高效液相色谱-电喷雾串联四极杆质谱
工业染料
果汁
葡萄酒
ultra performance liquid chromatography-electrospray ionization tandem quadrupole mass spectrometry （UPLC-ESI-MS/MS）
industrial dyes
juice
wine
