摘要
用直接进样（Ｃ１８柱）反相高效液相色谱法研究了Ｍｅｎ＋－Ｄｚ（二硫腙）体系的色谱行为，建立了同时测定Ｃｄ，Ｈｇ，Ｐｂ和Ｃｕ的分析方法。方法的线性范围为０．０１～２．０ｍｇ／Ｌ，最低检出质量浓度为２．４～５．０μｇ／Ｌ，相对标准偏差为１．８％～９．７％，回收率为９４％～１０３％（Ｈｇ除外）。直接进样反相高效液相色谱法比萃取进样正相液相色谱法更快速，更简便，更容易操作，已用于人发测定。
The chromatographic behaviors of Me n + Dz(dithizone) have been studied with RP HPLC. A method for the determination of Cd, Cu, Pb and Hg has been established. The chromatographic conditions were: column:Shim pack CLC ODS,150 mm×6 0 mm i.d.; mobile phase: V (methanol)∶ V (water)∶ V (chloroform)(containing volume fraction 1% triethylamine)=80∶12∶8; flow rate: 1 mL/min; column temperature:35℃;detection wavelength:254 nm. The linear ranges were from 0 01 mg/mL to 2 0 mg/mL with correlation coefficients of 0 999 3 0 999 8. The detection limits of Cd, Cu, Pb, Hg were from 2 4 μg/L to 5 0 μg/L. The RSD s were in the range from 1 8% to 9 7%,and the recoveries ranged from 94% to 103% (except Hg). The method has been applied to the analysis of hair.
出处
《色谱》
CAS
CSCD
北大核心
1998年第6期545-547,共3页
Chinese Journal of Chromatography
关键词
镉
汞
铅
铜
人发
HPLC
分析
reversed phase high performance liquid chromatography, Cd, Cu, Pb, Hg, hair
