摘要
研究了以甲醛活化的壳聚糖为载体固定化ｌ天冬酰胺酶，其活力回收可达到１１３％，比用以戊二醛为交联剂进行固定化的活力回收要高出五倍多。分别从缓冲液的ｐＨ值及甲醛用量等方面进行了固定化ｌ－天冬酰胺酶的条件优化，并且对固定化酶的性质作了探讨。
asparaginase was immobilized on chitosan activated by formaldehyde. Experiments showed that the activity retention could reach 113%, which was nearly five times the activity of immobilized l asparaginase by using glutaraldehyde as crosslinking reagent.The optimum immobilization conditions were obtained by investigating the buffer pH value and formaldehyde dosage. The properties of immobilized l asparaginase were also studied.
出处
《功能高分子学报》
CAS
CSCD
1998年第4期534-538,共5页
Journal of Functional Polymers
关键词
壳聚糖
天冬酰胺酶
固定化酶
抗白血病药
Chitosan,l Asparaginase,Immobilized enzyme, Immobilization
