摘要
目的探索氢氧化铝佐剂戊肝疫苗的免疫原性与疫苗中PO_4^(3-)浓度的关系,并分析抗体滴度与抗原在羊淋巴液中吸附率有无相关性。方法用不同浓度PO_4^(3-)处理氢氧化铝佐剂制备试验疫苗,腹腔免疫Balb/c小鼠,ELISA法定量检测免疫后不同时间血清抗体滴度,同时采用钼酸铵还原法检测试验疫苗离心沉淀中的磷含量,根据兰格缪尔方程计算抗原在含不同浓度PO_4^(3-)的佐剂表面的最大吸附量(Γm)。试验疫苗在羊淋巴液中的抗原吸附率采用ELISA法检测。结果随疫苗中PO_4^(3-)浓度增加,抗原最大吸附量(Γm)减小,抗原在羊淋巴液中的吸附率下降,免疫血清抗体滴度渐次增加,直至达到平稳状态。结论在一定范围内增加疫苗磷酸根含量可减小抗原在羊淋巴液中吸附率,降低佐剂对抗原的吸附程度,提高疫苗免疫抗体滴度。
To explore the relationship between phosphate （PO43-） and immunogenicity of HEV-aluminum hydroxide adjuvant vaccine, and to investigate the association of antibody titers with adsorption percentage of antigen in sheep lymph fluid. We prepared experimental vaccines with aluminum-hydroxide adjuvant, which has been pretreated with different quantity of phosphate. The experimental vaccine was used to immunize Balb/c mice intraperitoneally （i.p.）. Subsequently, the immune sera at different weeks were collected and the antibody titer was measured quantitatively by ELISA. Phosphorus in precipitate was determined by ammonium molybdate reduction assay after vaccine centrifuge. The adsorptive capacity （Fro） of antigen onto aluminum-hydroxide adjuvant was calculated according to the langmuir adsorption equation. And adsorption percentage of antigen was quantitatively detected by ELISA when experimental vaccines were mixed with sheep lymph fluid. As phosphate in vaccine increased, （Fro） of antigen lowered, and adsorption percentage of antigen decreased after the mix of vaccine with sheep lymph fluid, antibody titer of immune mouse sera raised gradually until steady state. The results suggest that increasing of phosphate content in certain extent of vaccine can decrease adsorption percentage of antigen, reduce antigen adsorption on aluminum adjuvant, but enhance antibody titer.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2010年第2期120-123,共4页
Immunological Journal
关键词
氢氧化铝佐剂
磷酸根
抗体滴度
吸附率
Aluminum hydroxide adjuvant
Phosphate
Antibody titer
Adsorption percentage
