摘要
目的:探讨水杨酸钠对顺铂导致的螺旋神经节细胞(SGNs)毒性的保护作用。方法:体外培养SGNs。分组:空白对照组,不同浓度水杨酸钠组、顺铂组和顺铂+水杨酸钠组(100～900μg/mL),加药48h后,通过显微镜下细胞计数及噻唑蓝(MTT)比色法对SGNs进行药物毒性检测,通过Hoechst33258进行细胞核染色,观察细胞凋亡情况。结果:顺铂(4、6、8μg/mL)对SGNs有明显毒性,促使细胞凋亡,并且随浓度增加,细胞数量明显减少,与空白对照组相比差异具有显著统计学意义(P<0.05)。一定浓度范围(≤500μg/mL)的水杨酸钠可以拮抗顺铂对SGNs的毒性,拮抗顺铂导致的细胞凋亡,与顺铂组相比差异具有显著统计学意义(P<0.05)。结论:在体外培养的条件下,水杨酸钠在一定浓度范围内可以拮抗顺铂导致的SGNs毒性,其保护机制可能与抑制细胞凋亡有关。
Objective To assess the protective effect of sodium salicylate on cisplatin-induced spiral ganglion neurons（SGNs） damage.Methods SGNs cultured in vitro were divided into several groups：Group A was a control group.Group B received different concentrations of sodium salicylate.Group C received different concentrations of cisplatin,and group D received cisplatin and different concentrations of sodium salicylate.After 48h,cells were counted under microscope and MTT assay was performed to test the cell cytotoxicity on SGN.Cell apoptosis was assessed by cell nucleus staining method.Results Cisplatin （4,6,8 μg/ml） had obvious cytotoxicity effect on SGN and enhanced cell apoptosis in a dose dependant manner,number of cell decreased markedly and was significantly lower than that of the blank controls （P 〈0.05）.Definite concentration （≥500 μg/ml） of sodium salicylate could antagonize the cytotoxicity and cell apoptosis induced by cisplatin,and significant differences were seen between the sodium salicylate protective group and the cisplatin group.Conclusions Sodium salicylate within a certain range of concentration had protective effect on cisplation induced SGN damage and this may be related to its anti-apoptosis effect.
出处
《诊断学理论与实践》
2010年第1期48-52,共5页
Journal of Diagnostics Concepts & Practice
基金
上海市教委基金资助项目(06BZ036)
关键词
顺铂
水杨酸钠
螺旋神经节细胞
细胞凋亡
Cisplatin
Sodium salicylate
Spiral ganglion neuron
Apoptosis
