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硼替佐米治疗小鼠重症急性胰腺炎 被引量:4

Treatment of acute pancreatitis in mice by PS-341
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摘要 目的 观察硼替佐米(PS-341)对小鼠重症急性胰腺炎(SAP)的治疗作用.方法 将40只ICR雌性小鼠随机分为PS-341治疗组(注射脂多糖前0.5 h腹内注射0.5 mg/kg PS-341)、模型对照组(注射脂多糖前0.5 h腹内注射50%DMSO)、空白对照组(生理盐水制模),采用连续7次腹内注射雨蛙素(每次间隔1 h)及脂多糖制作小鼠SAP模型.最后一次注射雨蛙素后2 h麻醉小鼠,自右颈静脉取血检测血淀粉酶(Amy)、乳酸脱氢酶(LDH)、C-反应蛋白(CRP);光镜下观察小鼠胰腺的病理形态,测定胰腺组织中髓过氧化物酶水平(MPO),实时荧光定量聚合酶链反应(PCR)测定胰腺组织中黏附分子(ICAM-1、P-selectin、E-selectin)的含量.结果 与模型对组比较,治疗组小鼠Amy、LDH、CRP明显下降(P〈0.05);治疗组小鼠胰腺的病理结果显示组织的炎细胞浸润及出血明显减少,胰腺组织中MPO和黏附分子的含量明显下降,两者之间差异有统计学意义(P〈0.05).结论 PS-341对小鼠SAP有一定的治疗作用,可望给临床上治疗SAP带来希望和前景. Objective To observe the therapeutic effects of PS-341 in the treatment of severe acute pancreatitis(SAP)in mice.Methods SAP was induced by cerulin and lipopolysaccharide(LPS)in mice.Thirty min before the administration of LPS,mice were treated with either PS-341 or vehicle.Serum amylase,C-reactive protein(CRP),and lactate dehydrogenase(LDH)were assessed,and tissue myeloperoxidase activity was measured.The expression of intercellular adhesion molecule 1,P-selectin,E-selectin in pancreas was detected by reverse transcriptase-polymerase chain reaction (RT-PCR).The pancreatic tissue samples were examined pathologically.Results Treatment with PS-341 significantly protected mice by attenuating myeloperoxidase activity,pancreatic morphological changes in histological sections,and down-regulating the mRNA expression of intercellular adhesion molecule 1,P-selectin,and E-selectin in pancreas as compared with vehicle-treated groups.Conclusion PS-341 may be a promising drug to prevent disease progression in SAP.
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2010年第1期26-28,I0001,共4页 Chinese Journal of Experimental Surgery
基金 浙江省科技厅资助项目(2006C33077)
关键词 重症急性胰腺炎 黏附分子 蛋白酶体抑制剂 Severe acute pancreatitis Adhesion molecule Proteasome inhibitor
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同被引文献29

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