摘要
目的探讨清胰汤对急性出血坏死性胰腺炎(AHNP)大鼠肠道细菌、内毒素移位的影响。方法SD大鼠随机分成AHNP组,AHNP+清胰汤治疗(QYT)组,假手术(SO)组。AHNP组与QYT组大鼠建立AHNP模型,SO组大鼠仅开腹翻动胰腺。QYT组造模后进行清胰汤胃灌注,另两组生理盐水灌胃,24 h后检测门静脉血内毒素(ET)浓度和大肠杆菌DNA含量(Ct值)。结果QYT组ET浓度、大肠杆菌DNA含量均低于AHNP组。结论清胰汤可降低实验性AHNP大鼠门静脉血中细菌DNA表达和内毒素浓度,减少肠道的细菌、内毒素移位。
Objective To study the effect of Qing Yi Tang（QYT） on bacterial and endotoxin translocation from intestine in acute hemorrhagic necrotizing pancreatitis（AHNP） in rats.Methods SD rats were randomly divided into 3 groups： AHNP group,AHNP ＋QYT treatment group and sham operation（SO） group.AHNP models were set up in the former two group,while the pancreas was only mobilized in SO group.After setting up the model,QYT was gavaged in the QYT group,while normal saline was gavaged in the SO group and AHNP group.The concentration of endotoxin（ET） and the DNA of E.coli expressoin in portal vein blood in three groups of rats were measured 24h later.Results The concentration of ET and amount of E.coli DNA in portal vein of QYT group were lower than those of AHNP group.Conclusions QYT can reduce significantly the concentration of ET and E.coli DNA in portal vein blood and decrease bacterial and endotoxin translocation from intestine in the AHNP rats.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2010年第3期255-258,共4页
China Journal of General Surgery
基金
湖南省科学技术厅课题资金资助项目(2008FJ3059)
关键词
胰腺炎
急性坏死性/治疗
清胰汤
细菌移位
大鼠
Pancreatitis
Acute Necrotizing /ther
Qing Yi Tang
Bacterial Translocation
Rats
