摘要
目的：研究柏氏禽刺螨在传播家鼠型肾综合征出血热病毒中的作用。方法：每罐放柏氏禽刺螨１００只，于室温为２３±１℃环境下饥饿４ｄ，分别叮刺感染汉坦病毒（Ｈａｎｔａａｎｖｉｒｕｓ）或浙４５株（Ｚ４５）大鼠乳小鼠２只或感染汉城病毒（Ｓｅｏｕｌｖｉｒｕｓ）ＵＲ株大鼠乳小鼠２只各１２ｈ，为感染螨，用感染后ｄ１５的螨叮刺正常大鼠乳小鼠９只，ｄ３０取感染鼠的肺和血清，用间接免疫荧光测试。于ｄ１５和ｄ２３取感染螨用聚合酶链反应测试其体内病毒ＲＮＡ。结果：柏氏禽刺螨分别叮刺感染汉坦病毒Ｚ４５株和感染汉城病毒ＵＲ株大鼠乳小鼠后，再分别叮刺正常大鼠乳小鼠５只和４只。Ｉ－ＦＡＴ检测结果汉坦病毒Ｚ４５株组的鼠全部阳性，汉城病毒ＵＲ株组的４只鼠中３只阳性，其抗体滴度，前者为１０－４０，后者为２０－４０，病毒在螨体内可保存２２ｄ以上。阻断试验显示，病毒免疫血清１∶２０作用肺切片标本后，部分阻断荧光抗体反应，与未经稀释的血清作用后全部阻断特异性荧光反应。对照组正常鼠肺片和血清经ＩＦＡＴ检测均阴性。
AIM: To demonstrate the role of rat mite ( Ornithonyssus bacoti ) in the transmission of Rattus borne hemorrhagic fever with renal syndrome (HFRS). METHODS: In the transmission experiments, about 100 O bacoti per pool were isolated and placed in a jar, unfed for 4 d at 23±1 ℃. Suckling Wistar rats inoculated with Hantavirus strain Z45 or Seoul virus strain UR were placed in each jar for free attack by the mites for 12 hours. After 14 d the normal suckling Wistar rats were bitten by the mites. Fifteen days later, the lung tissues and sera of the infected rats were collected and detected for Hantaviral antigen by indirect fluorescent antibody technique (IFAT). For demonstration of the infection of O bacti with Rattus borne Hantavirus PCR technique was applied to detect Rattus borne Hantaviral RNA. RESULTS:Sukling Wistar rats inoculted Hantavirus strain Z45 or Seoul virus strain UR were bitten by O. bacoti and then these mites were fed on 4 and 5 of normal suckling rats in each jar, respectively. The antigens of Hantavirus strain Z45 were postive in all the lungs of the normal rats bitten by the mites, the sera titers of the rats were from 1∶10 to 1∶40. The antigens were postive in 3 of the 4 rats, the sera titers were from 1∶20 to 1∶40. Both of the viruses could be maintained in O bacoti for 22 days. The blocking test showed when 1∶20 Hantavirus immunosera were exposed to the lung samples and then reacted with the sera from the patients with HFRS,all the specific fluorescence reactions of the samples were blocked,whereas the control group including the normal rat lung tissues and sera were all negative(Fig.1).CONCLUSION: O bacoti might play a role as the vector of HFRS and a reservoir host as well.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1998年第6期445-448,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家自然科学基金
关键词
柏氏禽刺螨
家鼠型汉坦病毒
叮刺传播
Ornithonyssus bacoti, Rattus borne hantavirus , biting transmission.
