摘要
目的研究无镁诱导惊厥后发育中皮层神经元表达糖皮质激素受体(glucocorticoid receptor,GR)的影响。方法以原代培养的胎鼠大脑皮层神经元无镁诱导的反复惊厥样放电为模型,根据对培养6d皮层神经元的不同处理分为正常细胞外液组和无镁细胞外液组。神经元在上述2种液体中孵育3h,然后恢复正常DMEM培养液继续培养,在无镁损害后1d、7d及12d时应用免疫细胞化学技术及实时定量PCR测定发育中大鼠皮层神经元无镁细胞外液处理后GR表达的变化。结果应用免疫细胞化学方法发现无镁损伤后12d惊厥组GR蛋白表达较对照组显著下降(P<0.01);实时定量PCR发现无镁损伤后1d、7d惊厥组GRmRNA表达较对照组显著增高(P<0.05)。结论无镁细胞外液处理可以诱导发育中大鼠皮层神经元GR表达的改变。
Objective To study the changes of glucocorticoid receptor （GR） expression in embryonic rat cortical neurons exposed to transient Mg~ 2＋ -free treatment.Methods Six days after rat cortical neuronal cultures, two groups were created based on the medium to which were transiently exposed. The control group was exposed to a physiological solution （PS）, and the Mg~ 2＋ -free group was exposed to the same medium as the control group except for the removal of magnesium. The expression of GR mRNA and protein was determined by real-time PCR and immunocytochemistry staining 1, 7 and 12 days after transient Mg~ 2＋ -free treatment. Results Compared to the control group, the Mg~ 2＋ -free group displayed the significantly less accumulated optical density （AOD） of GR immunoreactivity 12 days after transient Mg~ 2＋ -free treatment （P 0.05） . On the contrary, GR mRNA expression increased significantly 1 and 7 days after transient Mg~ 2＋ -free treatment in the Mg~ 2＋ -free group （P0.05）.Conclusions GR expression is modified following Mg-free-induced injury in cultured developing neurons in rats.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2010年第3期211-214,共4页
Chinese Journal of Contemporary Pediatrics
关键词
糖皮质激素受体
神经元
镁
胎鼠
Glucocorticoid receptor
Neuron
Magnesium
Fetal rats
