摘要
目的研究低功率聚焦超声联合微泡靶向开放兔血脑屏障（BBB）后其通透性变化规律。方法MRI引导下，静脉注射微泡，聚焦超声照射兔大脑一侧靶点，对侧镜像点为对照组，未处理者为健康组。照射后即刻、2hAh、6h、8h、24h,1周分别行MR]增强扫描测定信号强度值；荧光分光光度法检测兔脑组织伊文思蓝量（EB）。结果超声照射后BBB即刻开放，2hMRI信号增强值和脑组织EB量达到峰值，8h后恢复正常。靶点MRI信号增强值与脑组织EB含量的相关系数r=0.964。结论MRI引导下聚焦超声联合微泡能靶向可逆地开放BBB，MRI信号增强值在一定程度上可反映BBB的开放状况。
Objective To explore the dynamic changes of blood brain barrier（BBB） permeability after MRI guided targeted BBB disruption by low- intensity focused ultrasound combined with microbubble in rabbit brain. Method After ultrasound contrast - agent（SonoVue） was injected in vein, MRI -guided focused ultrasound was applied at target location in one bemisphere of rabbits＇ brains to induce BBB disruption. The opposite side was used as control group. MRI enhancedment scanning was applied before sonication at 0 h,2 h,4 h,6 h,8 h,24 h, 1 week after sonication and the signal intensity was measured. BBB permeability in target was assessed at 0 h,2 h,4 h,6 h, 8 h, 24 h, 1 week after sonication by fluoresence spectrophotometer for Evans Blue （EB） extravasation. Results BBB permeability change was occured immediately after sonication. The MRI signal intensity and the EB extravasation peaked at 2 h（ P 〈0.05） and decreased to normal level at 8 h after sonication. The changes of MRI signal intensity was correlated with the EB extravasation. Their coefficient correlation was 0. 964 （ P 〈 0. 05 ）. Conclusions MRI -guided focused ultrasound could disrupt BBB selectively and reversibly. The changes of MRI signal intensity could evaluate the situation of BBB disruption quantitatively.
出处
《中华神经外科杂志》
CSCD
北大核心
2010年第3期277-280,共4页
Chinese Journal of Neurosurgery
基金
基金项目：国家自然科学基金（30870723）
关键词
血脑屏障
超声检查
毛细血管通透性
磁共振成像
Blood - brain barrier
Ultrasound
Capillary permeability
Magnetic resonance imaging
