摘要
目的:对石斛多糖提取工艺进行优化。方法:比较不同浸提温度、时间和除蛋白方法对多糖得率的影响,优化工艺。结果:石斛多糖最佳提取工艺条件为90℃水浸提3～4h,料水体积比为1∶50,采用Sevag法脱蛋白,样品-Sevag溶液=1∶1、氯仿-正丁醇=10∶2.5,提取时间为30min。结论:此方法提取石斛多糖得率高,为理想的提取条件。
Objective：To study optimum extracted-conditions of polysaccharide in Denderobiun. Methods：The most yield of polysaccharide was gotten which volume ratio of dendrobium to water,distilling temperaturethe and method of isolating protein were used. Results：The effects was best when volume ratio of dendrobium to water was 1∶50,distilling at 90 ℃ for 3-4 hours,isolating protein by seveg method in the mixture composed of 50% sample and 50% sevag solution,the ratio of chloroform and n-butano was 10∶2.5,the extraction technology was 30 min. Conclusion：The extraction method is perfect because the most yield of polysacchareide is gotten.
出处
《中国医药导报》
CAS
2010年第12期40-41,43,共3页
China Medical Herald
关键词
石斛
多糖
提取工艺
Dendrobium
Polysaccharide
Extraction technology
