摘要
目的:建立高效液相法测定腰息痛丸中芍药苷含量的方法。方法:采用Thermo ODS-2Hypersil(4.6mm×150mm,5μm)色谱柱;流动相:乙腈-0.1%磷酸(13∶87);检测波长:230nm;流速:1.0ml/min。结果:替加氟在0.04～0.88μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率为101.50%,RSD=1.20%。结论:本方法简便、准确、重现性好,可用于腰息痛丸中芍药苷的质量控制。
Objective：To establish a method for the content determination of paeoniflorin in Yaoxitong Pills by HPLC. Methods：The Thermo ODS-2 Hypersil （4.6 mm×150 mm,5 μm） column was used. The mobile phases consisted of acetonitrile and 0.1%-phosphoric acid （13∶87）,the flow rate was 1.0 ml/min and the detection wavelength was at 230 nm. Results：The linear ranges of paeoniflorin was at 0.04-0.88 μg （r=0.999 9）,the average recovery was 101.50% with a RSD of 1.20%. Conclusion：The method is fast,reliable,accurate and can be used in the quality control of paeoniflorin in Yaoxitong Pills.
出处
《中国医药导报》
CAS
2010年第12期70-71,共2页
China Medical Herald
