摘要
目的:研究超声技术提取葛根中葛根素的最佳条件,建立高效液相色谱法测定葛根中葛根素含量的方法。方法:采用超声技术提取葛根素,并利用正交试验法进行优选超声提取葛根素的最佳试验条件,采用高效液相色谱法,以葛根素为化学对照品,波长:250nm测定葛根中葛根素含量。结果:各因素对葛根素提取率的影响次序为:超声次数(超声时间(甲醇加入量(甲醇浓度。葛根素回归方程为:A=-152791.51+868038.61M,r=0.9997。精密度试验RSD%=1.0,仪器精密度良好;稳定性试验RSD%=1.3,在5h测定葛根素的含量是稳定的;重现性试验RSD%=2.39、平均回收率101.0%;结论:超声提取法提取效率高、操作简便、省时,是提取葛根有效成分的较好方法,值得推广。
Objective：To research the best method of extracting Puerarin from Pueraria.Establishing the method of determining the content of puerarin in Pueraria by HPLC.Methods：Extraction Puerarin by ultrasonic technology.The optimum extraction process by ultrasonic was the yielding contents of puerarin in extract which were determined by HPLC,puerarin for the standard,wavelength：250nm.Results：The influence order of each factor on the extracting of puerarin was that the extracting times﹥extracting time﹥methanol quantity﹥concentration of methanol.The return formula of puerarin is A=-152791.51＋868038.61M,r=0.9997,Precision RSD=1.0%,equipment in good precision,Stability RSD=1.3%,determining the content of puerarin in 5 hours by HPLC is stable.repeatability RSD=2.39%,the average recovery rate of 101.0%.Conclusion：The ultrasonic extraction method is of high extracting efficiency,simple and time-saving.Which is a good method to extracte the active ingredients of Radix Puerariae lobatae,it is worth promoting.
出处
《辽宁中医杂志》
CAS
北大核心
2010年第4期700-702,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
四川省卫生厅科研项目(川卫办发[2006]437号)
关键词
葛根
葛根素
超声技术
正交试验
含量测定
高效液相色谱法
紫外色谱法
Radix Puerariae Lobatae
Puerarin
Ultrasonic technology
Orthogonal tests
Determine the cintent of puerarin
HPLC
UV--vis
