摘要
以瑞特姆油葵无菌苗的茎尖、下胚轴、子叶、真叶为外植体,1/2MS培养基为基本培养基,研究不同激素水平对油葵愈伤组织形成和芽再生及生根的影响。结果表明:KT激素诱导的愈伤组织多为蓬松的绿色,芽再生率相对较高;外植体中破坏生长点的茎尖再生率最高;诱导芽的最佳配方为:MS+KT0.5 mg/L+NAA0.2 mg/L,芽分化率可达75%;理想的生根培养基的配方为:1/2MS+IBA0.5 mg/L,生根率达到80%。
The apical shoot broken, hypocotyl, cotyledon, leaf from sterile seedling were cultured in MS medium with different hormones. The result showed that more fluey green callus were gotten and some buds were regenerated from the callus with treatment of KT. The apical shoot broken among explants regenerated best and its differentiation ratio was 75% in the medium of MS＋KT 0. 5 mg/L＋NAA 0. 2 mg/L. The best rooting medium was 1/2MS＋IBA 0. 5 L in which the rooting ratio was 80%.
出处
《北方园艺》
CAS
北大核心
2010年第7期124-126,共3页
Northern Horticulture
基金
天津市科委科技支撑计划资助项目(08ZCKFNC01200
07ZCKFNC01100)
关键词
油葵
瑞特姆
再生
破坏生长点的茎尖
oil sunflower
Ritmo
regeneration,
apical shoot broken
