摘要
目的:建立桔梗药材的HPLC指纹图谱并测定桔梗皂苷D的含量。方法:采用RP-HPLC法,以Hypersil C18柱(250mm×4.6 mm,5μm)为色谱柱,乙腈-0.05 mol/L H3PO4溶液进行梯度洗脱,流速0.5 mL/min,检测波长210 nm(桔梗皂苷D含量测定为206 nm),柱温30℃。结果:以桔梗皂苷D为参照物峰,确定了12个共有峰,建立了18批药材的共有图谱,并比较了样品中桔梗皂苷D的含量。结论:所建立的HPLC指纹图谱及含量分析方法均具有较好的重现性,两者结合为桔梗药材质量控制提供了新的方法。
AIM： To establish HPLC-fingerprints and quantitatively determine platycodin-D from Platycodon grandiflorum.METHODS： HPLC analysis was carried out on Hypersil C18 column（250 mm × 4.6 mm,5 μm）,with a mobile phase of acetonitrile-0.05 mol/L phosphoric acid system,gradient elution,with a flow at 0.5 mL/ min,an ultraviolet detection wavelength was at 210 nm for fingerprint and at 206 nm for platycodin-D,column tem-perature at 30 ℃.RESULTS： Twelve common peaks were identified in chromatograms with reference to platycod-in-D peak from the 18 batches of the samples.CONCLUSION： The method of the HPLC-fingerprint and quantita-tive analysis is rapid,simple and accurate with a good reproducibility and can be used for the quality control of Platycodon grandiflorum.
出处
《中成药》
CAS
CSCD
北大核心
2010年第4期529-532,共4页
Chinese Traditional Patent Medicine
基金
河南省科技厅资助项目(0323032200)
关键词
桔梗
指纹图谱
桔梗皂苷D
含量测定
Platycodon grandiflorum
fingerprint
platycodin-D
determination
