摘要
【目的】观察慢性乙型肝炎(慢乙肝)患者核苷类似物治疗后调节性T细胞的频率。【方法】用流式细胞仪检测患者的CD4+CD127loCD25hi-int调节性T细胞。比较经核苷类似物治疗后慢乙肝CD4+CD127loCD25hi-int调节性T细胞的频率。【结果】经核苷类似物治疗后无效组Treg高于有效组与健康对照组,差别有统计学意义(P=0.004,P=0.000);有效组与健康组之间差别无统计学意义(P>0.05)。经核苷类似物治疗后,慢乙肝患者不同水平转氨酶之间Treg的频率无差别(P>0.05)。经核苷类似物治疗后,高病毒载量组Treg的水平高于低病毒载量组及HBV-DNA转阴组(P值分别为0.002、0.039),但转阴组与病毒低载量组之间比较Treg无差别(P>0.05)。经核苷类似物治疗后病毒是否发生变异组间Treg水平的差别无统计学意义(P>0.05)。经核苷类似物治疗后是否实现HBeAg血清学转换之间比较Treg,差别无统计学意义(P>0.05)。【结论】慢乙肝患者的Treg能够抑制机体特异的抗病毒的免疫反应,经核苷类似物治疗后Treg的下降与低表达可能导致慢乙肝患者免疫功能的部分恢复,以利于机体内病毒的清除。故检测患者的Treg再结合其它免疫指标将为初步预测核苷类似物抗病毒疗效提供帮助。
[Objective] To study the level of regulatory thymus cell in patients with chronic hepatitis B virus infection after nucleoside analogues＇ treatment. [Methods] CD4＋CD127loCD25hi-int regulatory T cell （Treg） was tested by Flow Cytometry. The levels of Treg in patients with chronic hepatitis B virus infection after nucleoside analogue treatment were compared. [Results]Treg level in ineffective patient group was significantly higher than effective patients after nucleoside analogue treatment and healthy control group （P = 0.004,P = 0.000）. There was no significant differences between effective patient group and healthy control group （P0.05）. Treg of inefficacious patients under different transaminase did not show obvious difference（P0.05）. Treg of the no significantly decreased group of the viral load in blood after Nucleoside Analogue treatment was higher than the low viral load in blood and the group HBV became negative,showing significant difference （P = 0.002,P = 0.039,respectively）,but Treg of the low viral load after Nucleoside Analogue treatment did not show obvious difference （ P0.05）. Treg of virus mutation group and no virus mutation group did not show obvious difference（P0.05）. Treg of HBeAg seroconversion group and no HBeAg seroconversion group did not show obvious difference（P0.05）.[Conclusions]Treg of chronic hepatitis B virus can inhibit antiviral immune response,so the high expression of Treg was correlated with the high viral load in chronic hepatitis B virus infection. The low expression and decrease of Treg could result in partial recovery of immune function with chronic hepatitis B virus infection after nucleoside analogue treatment,so it would be beneficial to viral clearance. Detection of Treg and other immune index would provide help for preliminary prediction efficacy of nucleoside analogue treatment.
出处
《武警医学院学报》
CAS
2010年第4期259-262,共4页
Acta Academiae Medicinae CPAPF
基金
天津市应用基础研究计划面上项目(06YFJMJC13100)
