摘要
目的探讨IL-6启动子-174G/C和-572C/G的单核苷酸多态性频率分布与2型糖尿病患者下肢血管病变的关系。方法 280例中国北方地区汉族成年2型糖尿病患者,根据临床诊断分为下肢血管病变(VDLE)组、下肢神经病变(NDLE)组、下肢血管-神经病变(VNDLE)组和无下肢并发症(NC)组,另选70例社区查体正常成人作为对照(CON)组。采用PCR-RFLP法测定IL-6启动子区-174G/C和-572C/G单核苷酸多态性;采用ELISA法测定血清IL-6、IL-6受体和hsCRP。结果 IL-6启动子的-174G/C基因型及等位基因在2型糖尿病组和对照组的频率分布差异均无统计学意义。而IL-6启动子-572C/G在2型糖尿病组中的基因型GG和CG、等位基因G均高于对照组(P<0.05),并且VDLE组和VNDLE组高于其他组(P<0.05)。结论 2型糖尿病下肢血管病变与IL-6启动子的-174G/C单核苷酸多态性可能无关,而与IL-6启动子区-572C/G单核苷酸多态性可能有关。IL-6启动子区-572C/G的GG基因型和G等位基因可能是2型糖尿病发生下肢血管病变的易患基因。
Objective To investigate the single nucleotide polymorphism of IL-6 promoter -174 G/C and -572C/G in type 2 diabetic patients and their association with lower extremity vascular disease. Methods Based upon the clinical manifestation and the ultrasound test of the lower extremities, 280 type 2 diabetic patients were divided into four groups: Vascular disease (VDLE) group, Neuropathic disease (NDLE)group, Vascular and Neuropathic disease(VNDLE) group, and no complication (NC) group.And 70 healthy people were recruited as control (CON). By PC R-RFLP technique, the polymorphism of the human IL-6 gene-174 G/C and -572C/G was evaluated.Also, the concentration of serum IL-6, IL-6 receptor and hs-CRP were measured by ELISA technique. Results There was no statistical difference Of the IL-6 promoter-174G/C genotypes and alleles in the type 2 diabetes mellitus group and CON group (P〉0.05). The genotype GG and CG and the allele G of IL-6 promoter-572C/G seemed to be more frequent in VDLE and VNDLE group than that in NC group as well as in the CON group (P〈0.05). Conclusion The polymorphism of the IL-6 promoter-572C/G but not the -174 G/C might be associated with the type 2 diabetes mellitus with low-extremity vascular disease, while the genotype GG and allele G of the IL-6 promoter -572C/G might be the susceptibility genes.
出处
《中国慢性病预防与控制》
CAS
北大核心
2010年第2期156-160,共5页
Chinese Journal of Prevention and Control of Chronic Diseases
关键词
糖尿病
2型
下肢血管病变
白细胞介素-6
多态性
Type 2 diabetes mellitus
Low-extremity vasculopathy
Interleukin-6
Polymorphism