摘要
目的:建立高效液相色谱法同时测定七味葡萄散中桂皮醛和甘草酸含量。方法:采用Agilent ZORBAX Eclipse SB-C_(18)(4.6 mm×150 mm,5μm)色谱柱,柱温为30℃;流动相为乙腈-3.2%冰醋酸(31:69);流速为1 mL·min^(-1);检测波长变换程序:0～15 min为285 nm,15～30 min为250nm。结果:桂皮醛和甘草酸保留时间分别约为10.4和20.9 min,与各自相邻峰的分离度均大于1.5。以峰面积对进样浓度(μg·mL^(-1))线性回归,桂皮醛回归方程:Y=0.01049X-2.914,r=0.9998,线性范围19.40～242.5μg·mL^(-1),甘草酸回归方程:Y=0.1409X+0.8962,r=0.9998,线性范围12.48～156.0μg·mL^(-1)。桂皮醛和甘草酸的回收率分别为98.4%和102.1%,RSD分别为1.3%和0.94%。结论:本方法操作简便,测定结果准确,重复性好,可用于七味葡萄散中桂皮醛和甘草酸的含量测定。
Objective：To establish an HPLC quantitative method for the determination of cinamaldehyde and glycyrrhizic acid in Qiwei Putao powder simultaneously.Methods：The chromatographic conditions include Agilent ZORBAX Eclipse SB -C18（4.6 mm×150 mm,5μm） column,acetonitrile - 3.2%glacialacetic acid（31：69） as mobile phase;The flow rate was 1 mL·min^-1 and monitored at 285 nm（1 -15 min）,250 nm（15.1 -30 min）. Results：The retention time of cinamaldehyde and glycyrrhizic acid was about 10.4 min and 20.9 min respectively. The resolution was more than 1.5.The regress equation for cinamaldehyde was Y = 0.01049X -2.914,r =0.9998, and the linear range was 19.40 -242.5μg·mL^-1.Glycyrrhizic acid was Y = 0.1409X ＋0.8962,r =0.9998,and the linear range was 12.48 - 156.0μg·mL^-1 The average recovery of cinamaldehyde and glycyrrhizic acid was 98.4%and 102.1%,RSD 1.3%and 0.94%,respectively.Conclusion：This method is simple,time - saving and accurate.It can be used for routine analysis of cinamaldehyde and glycyrrhizic acid in Qiwei Putao powder.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第4期700-702,共3页
Chinese Journal of Pharmaceutical Analysis
