摘要
目的:采用高效液相色谱法对21批不同产地紫花地丁药材中秦皮乙素的含量进行了测定。方法:使用Thermo HYpersil-keystong BDS C18高效液相色谱柱,以甲醇-0.1%磷酸(15∶85)为流动相,检测波长349 nm。结果:不同产地的紫花地丁中秦皮乙素的含量有较大差异,其中河北产紫花地丁中秦皮乙素的含量较高,但不稳定;陕西、河南产紫花地丁中秦皮乙素的含量较稳定。结论:紫花地丁中秦皮乙素的含量随地域差异变化较大。
Objective:To compare the contents of esculetin in Viola yedoensis Makino.from Different Producing Regions.Methods:HPLC method was used.The HPLC system consisted of ThermoHYpersil-keystong BDS C18 column(150mm×4.6mm 5μm) and methanol-0.1% Phosphorylation(15:85) mixture as the mobile phase.The detection wavelength was at 349 nm.Results:The content of esculetin in Viola yedoensis Makino.from different producing regions is remarkably different.Conclusion:Producing regions is the crucial factor for quality of processing and producing in Viola yedoensis Makino.
出处
《成都中医药大学学报》
2010年第1期72-73,96,共3页
Journal of Chengdu University of Traditional Chinese Medicine
基金
四川省中医药管理局科研基金资助项目(编号:200613)
关键词
紫花地丁
秦皮乙素
含量
Viola yedoensis Makino
Esculetin Determination