摘要
目的:通过血清高效液相色谱(HPLC)指纹图谱法考察芍药甘草汤伍用的合理性。方法:比较单味药与复方水煎液及单味药与复方含药血清HPLC指纹图谱,确定单味药和组成复方后入血成分数与量的变化。结果:白芍(S)单味药有8个入血成分,甘草(G)单味药有23个入血成分,伍用后有32个入血成分;其中白芍苷(SGT6)、芍药苷(SGT7)、新甘草苷(SGT10)、甘草苷(SGT11)、SGT14、SGT15、SGT16、SGT17、SGT18、SGT24、SGT25、SGT26、SGT27、SGT28、甘草酸(SGT29)、甘草次酸(SGT32)伍用后峰面积明显增加。结论:白芍、甘草配伍后,两者生物利用度明显提高,存在相互协同作用。采用血清HPLC指纹图谱法证明了芍药甘草汤组方配伍的科学合理性,方法简便、准确、稳定。
OBJECTIVE：To study the compatible rationality of Shaoyao gancao decoction by serum HPLC fingerprint. METHODS：The serum HPLC fingerprints of Paeonia lactiflora,Glycyrrhiza uralensis and Shaoyao gancao decoction were compared to determine the number and quantity change of their component in serum. RESULTS：There were eight components in serum of P. lactiflora,23 components in serum of G. uralensis and 32 components in serum of Shaoyao gancao decoction. Some peak areas of serum samples were obviously increased,such as SGT6,SGT7,SGT10,SGT11,SGT14,SGT15,SGT16,SGT17,SGT18,SGT24,SGT25,SGT26,SGT27,SGT28,SGT29 and SGT32. CONCLUSION：The bioavailability is obviously improved after combination of P. lactiflora with G. uralensis,which shows synergism between them. Serum HPLC fingerprint certify the compatible rationality of combination of P. lactiflora and G. uralensis. Established method is simple,accurate and stable.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第15期1347-1350,共4页
China Pharmacy
