摘要
目的探讨宫颈上皮内瘤变（CIN）和宫颈癌细胞涂片内人类染色体端粒酶基因（hTERC）扩增情况及其临床意义。方法2007年12月至2008年12月收集济南军区总医院107例宫颈脱落上皮标本,包括对照组20例;研究组87例,其中27例CIN（Ⅰ级8例、Ⅱ~Ⅲ级19例）和45例宫颈鳞癌（原位癌7例、Ⅰ期14例、≥Ⅱ期24例）,术后2年受访15例（宫颈电热圈环切术治疗后12例,宫颈癌术后3例）。应用双色间期荧光原位杂交（FISH）技术,检测上述标本的hTERC基因扩增情况,并与液基薄层细胞检测（TCT）和病理学对照。结果（1）由对照组得出正常值为≤6.0%,〉6.0%即为阳性。（2）27例CIN：Ⅰ期出现hTERC基因扩增阳性细胞百分数为9.17±4.62,阳性率为50.0%（4/8）;CINⅡ~Ⅲ期为16.00±14.44,阳性率为84.2%（16/19）;原位癌为19.67±13.26,阳性率为85.7%（6/7）。CIN组、原位癌组和浸润型癌组hTERC基因扩增阳性细胞百分数均显著高于对照组（P〈0.01）;CINⅠ期组和Ⅱ~Ⅲ组、原位癌组、Ⅰ期宫颈浸润型鳞癌组和≥Ⅱ期组之间hTERC基因扩增阳性细胞百分率递次增高,差异有统计学意义（P〈0.05）。结论对于CIN,FISH检测宫颈上皮hTERC基因扩增是目前较新型的无创筛查方法,特别是对于辨别CIN是否具备恶性进展潜能具有重要意义。
Objective To investigate the genomic amplification of the human telomerase gene （hTERC） in cytogic specimen by fluorescence in situ hybridization （FISH） for the diagnosis of cervical dysplasia and cervical cacinoma.Mathods One hundred and senven cases of cervical cytology specimens in pap-smears, 20 cases with negtive for intraepithelial lesion or malignancy （NILM） for control group, 87 cases of study group including 8 with CIN I stage, 19 with CIN Ⅱ/Ⅲ, 7 with cacinoma in situ, 14 with invasive-type of squamous cacinoma in I stage, 24 with ≥Ⅱ stage and 15 followed-up cases after treatment by LEEP（Loop Electrosurgical Excision Procedure）（12 cases） and radical surgery（3 cases） were detected their hTERC genomic amplification by dual-color interphase FISH, and compared with cytologic （TCT） and histologic results. The pathologic result is the fainal diagnosis. Results （1）The diagnostic standard from the control group was ≤6.0%,6.0% meaned positive. （2）The genomic amplification rates of hTERC and positive percent rates were found 9.17±4.62 and 50.0%（4 /8） in CIN Ⅰ;16.00±14.44 and 84.2%（16/19）in CIN Ⅱ~Ⅲ;19.67± 13.26 and 85.7%（6 /7）in cacinoma in situ. There was statistically significant difference between in the control and study groups（P0.01）, and among in the CIN Ⅰ, CINⅡ~Ⅲ, cacinoma in situ,I stage and in ≥Ⅱ stage groups（P0.05）. Conclusion The investigation of the genomic amplification of hTERC is a better marker in present to screen cevical cytological specimens for CIN, espicially in differentiating its maligancy.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2010年第3期201-204,共4页
Chinese Journal of Practical Gynecology and Obstetrics
基金
国家卫生部科研基金项目(WKJ2007-3-001)
关键词
荧光原位杂交
宫颈上皮内瘤变
宫颈癌
端粒酶
fluorescence in situ hybridization
cervical intraepithelial neoplasia
cervical cacinoma
telomerase
