摘要
利用4对AFLP引物首次对我国北方沿海主要的6个栉孔扇贝养殖区的自然采苗群体进行了遗传多样性分析,共扩增得到191条清晰带谱,其中175条具有多态性。对各群体进行遗传多样性分析,结果表明各群体均具有较高的遗传多样性水平,香农式多样性指数在0.347 4～0.391 7之间。AMOVA结果表明遗传变异主要来源于群体内,占总遗传变异的87.22%。个体聚类结果表明,烟台、长岛、大连3个群体的个体聚在一起,可看做1个群体;而荣成群体的个体单独聚在一起,具有独立的遗传结构;胶南和日照的一部分个体聚在一起,其他大部分各群体单独聚在一起;推测是海洋环境和人工养殖影响的共同结果。
The AFLP technique was used to analyze the genetic diversity of 6 natural populations of Zhikong scallop（Chlamys farreri） from the main cultured zones along the northern coastline of China.Based on 4 pairs of primers,191 clear and repeated DNA fragments were generated,of which 175 were polymorphic.The genetic diversity analysis of the populations showed high levels of genetic diversity of 6 populations,and the Shannon s genetic diversity index varied from 0.347 4 to 0.391 7.The AMOVA（an-alyzing molecular varaiance） analysis indicated 87.22% viaration were within populations.The phylogeny tree showed that the individuals of Rongcheng clustered together,and had formed its own dependent ge-netic structure.The Dalian,Changdao and Yantai populations formed a cluster that could be regarded as one population.Most of the individuals of Jiaonan and Rizhao populations clustered respectively,but a few individuals clustered together.The structure of the populations of Zhikong scallop along the nortern coastline of China may have resulted from the oceanic environment and the interference of artificial cul-ture.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第4期38-42,共5页
Periodical of Ocean University of China
基金
国家高技术研究发展计划重大项目(2006AA10A408)资助
