摘要
目的研究玉屏风散提取液对小鼠腹腔巨噬细胞增殖及活化的影响。方法分别制备玉屏风散水煎液、玉屏风散蒸馏水超声提取液、玉屏风散无水乙醇超声提取液和玉屏风散乙酸乙酯超声提取液。分离健康昆明种小鼠原代腹腔巨噬细胞进行体外培养,培养基中分别加入不同剂量上述药液使终末浓度达到1.67、3.33、8.33、16.67 g·L^(-1)(折合生药)进行细胞培养,以0 g·L^(-1)作为空白对照组。培养24、72 h后,分别加入中性红、2-(2-甲氧基-4-硝基苯基)-3-(4-硝基苯基)-5-(2,4-二磺酸苯)-2H-四唑单钠盐(WST-8),用酶标法检测4种玉屏风散提取液对腹腔巨噬细胞活化及增殖的影响。结果与空白对照组相比,4种不同浓度的玉屏风散提取液均可促进小鼠腹腔巨噬细胞吞噬中性红能力及增殖(P<0.01)。与同剂量玉屏风散水煎液相比,除玉屏风散无水乙醇超声提取液(3.33 g·L^(-1))、玉屏风散乙酸乙酯超声提取液(3.33 g·L^(-1))可促进巨噬细胞吞噬中性红能力(P<0.05,P<0.01),玉屏风散乙酸乙酯超声提取液(16.67 g·L^(-1))可促进腹腔巨噬细胞增殖(P<0.01),其余剂量的玉屏风散超声提取液的效果都不如同剂量组的玉屏风散水煎液。结论玉屏风散提取液可促进小鼠腹腔巨噬细胞活化吞噬能力及增殖,但玉屏风散的超声提取条件需进一步探索。
Objective To study the effects of jade screen powder on proliferation and activation of peritoneal macrophages in mice. Methods Decoctum of distilled waters, supersound extract of distilled waters, supersound extract of dehydrated alcohol, supersound extract of acetidin of jade screen powder were prepared respectively. Primary peritoneal macrophages in healthy Kun- ming mice were separated and cultured in vitro. Jade screen powder of different doses were added in medium in order to make fi- nal concentration achieve 1.67,3.33,8.33 and 16.67 g · L-1（ convert into crude drug） ,0 g · L-1 as the control group. Neutral red and 2- （2-methoxyl-4-nitrophenyl） -3- （ 4-nitrophenyl ） -5- （ 2,4-sulfonic acid benzene） -2H-tetrazolium monosodium salt （ WST- 8 ） were added after cultured for 24 and 72 hours, the activation and proliferation of peritoneal macrophages were detected by en- zyme linked immunosorbent assay. Results Compared with the control group, different concentrations of jade screen powder can promote the proliferation and the capability of phagocytizing neutral red of peritoneal maerophages（ P 〈 0.01 ）. Except the supersound extract of dehydrated alcohol of jade screen powder（3.33 g · L-1 ） and supersound extract of acetidin of jade screen pow- der（ 3.33 g · L-1） can promote the capability of phagocytizing neutral red of macrophage （P 〈 0.05,P 〈 0.01 ）, and supersound extract of acetidin of jade screen powder（ 16.67 g · L-1 ） can promote the proliferation of macrophage（P 〈 0.01 ） ,the other con- centrations of supersound extract were not better than the same concentration of decoctum of distilled waters. Conclusion Jade screen powder can promote the proliferation and the capability of phagoeytizing of peritoneal macrophages, but the way of super- sound extraction of jade screen powder should be further explored.
出处
《新乡医学院学报》
CAS
2010年第3期244-247,共4页
Journal of Xinxiang Medical University
基金
河南省科技攻关项目(编号:0424420037)
关键词
玉屏风散
腹腔巨噬细胞
增殖
吞噬
jade screen powder
peritoneal macrophages
proliferation
phagocytosis
