摘要
目的探讨短期丰富生存环境干预对中老年雌性大鼠海马结构及其内有髓神经纤维的影响。方法将20只14月龄的雌性SD大鼠随机分为丰富生存环境组和标准环境组,每组各10只,对丰富生存环境组的动物给予4个月丰富生存环境干预,标准环境组于普通标准环境下饲养4个月;然后每组各随机选取5只,采用Morris水迷宫对大鼠的空间学习能力进行测试,然后运用透射电子显微镜和体视学方法分别对大鼠大脑海马结构及其内有髓神经纤维进行定量研究。结果短期丰富生存环境组中老年雌性大鼠与标准环境组相比,其空间学习能力明显增强;丰富生存环境组海马结构内有髓神经纤维总长度和总体积分别显著增加了43.4%和47.4%,且有髓神经纤维总长度的增加主要是由于细小直径的有髓神经纤维长度增加所致。海马结构总体积和海马结构内有髓神经纤维直径未见改变。结论4个月丰富生存环境干预对于14月龄雌性大鼠空间学习能力、海马内有髓神经纤维均有显著性影响。
Objective To investigate the effects of shortterm enriched environment on the hippocampal formation and the myelinated fibers in the hippocampal formation of midaged female rats. Methods Twenty 14month female SD rats were randomly divided into 10 enriched environment （EE） rats and 10 standard environment （SE） rats. EE rats were reared in enriched environment and SE rats were reared in standard environment for 4 months. Then, five rats were randomly selected from each group. The spatial learning capacity was assessed with Morris water maze. The hippocampal formation and the myelinated fibers in the rat hippocampal formation were quantitatively investigated with transmission electronic microscopy technique and stereological methods. Results Short-term enriched environment enhanced the spatial learning capacity of the mid-aged female rats. The total length and total volume of the myelinated fibers in the hippocampal formation of the EE rats was significantly increased by 43.3% and 47.4%, respectively, when compared to the SE rats. There was no significant difference in the hippocampal volume and the mean diameter of the myelinated fibers in the hippocampal formation between two groups. The increase of the total length of the myelinated nerve fibers in the hippocampal formation was mainly due to the increase of the myelinated fibers with small diameter. Conclusion Short-term enriched environment had significant effects on the spatial learning capacity and the myelinated fibers in the hippocampal formation of middleaged female rats.
出处
《解剖学报》
CAS
CSCD
北大核心
2010年第2期219-223,共5页
Acta Anatomica Sinica
