摘要
目的通过研究高压氧治疗对颅脑损伤大鼠脑组织水通道蛋白-4（AQP-4）表达与脑水肿的影响．探讨高压氧治疗颅脑损伤的可能作用机制。方法将雄性SD大鼠按随机数字表法分成3组，即假手术组、外伤对照组和高压氧治疗组，其中外伤对照组和高压氧治疗组均分为致伤后12h、1d、3d及5d4组。采用自由落体法制作颅脑损伤模型。干湿比重法测定脑组织的含水量，免疫组化法测定AQP-4的表达。结果高压氧治疗组脑组织含水量较外伤对照组明显减少，但较假手术组增多．比较差异均有统计学意义（P〈0．05）。AQP-4在各组大鼠脑组织中均有表达，假手术组呈低表达：颅脑损伤后12h伤灶周围水肿区星形胶质细胞足突AQP-4表达开始增高，1d达到高峰。3d后降低：高压氧组治疗大鼠各时间点伤灶周围AQP-4的表达与外伤对照组相比明显减低，差异具有统计学意义（P〈0．05）。结论高压氧治疗可能通过AQP-4表达的减少来减轻脑水肿的发生。从而保护病灶周围脑组织。
Objective To explore the effect of hyperbaric oxygen therapy （HBOT） on the expression of AQP-4 and the brain edema to discuss the possible mechanisms of HBOT in the treatment of trauma brain injury. Methods Male Sprague-Dawley rats were randomized into sham-operated group, trauma control group and HBOT group （n=32）; and then, the later 2 groups were subdivided into 4 groups according to the injury times （12 h, 1, 3 and 5 d）, respectively （n=8）. Feeney＇s brain injury models were induced; the water content of the brain tissue was measured by dry-wet method; the expression of AQP-4 was detected by immunohistochemical staining. Results The water content of the brain tissue in the HBOT group was significantly decreased as compared with that in the trauma control group, but significantly increased as compared with that in the sham-operated group （P〈0.05）. The protein expression level of AQP-4 was noted in all the brain tissues with the lowest level appearing in the sham-operated group. Increased protein expression of foot process AQP-4 was found in astrocyte cells of the contusion marginal region in the trauma control group 12 h after the operation, reaching its summit at ld and starting to decrease at 3 d. The expression of AQP-4 in the HBOT group was decreased significantly as compared with that in the trauma control group at each time points （P〈0.05）. Conclusion HBOT may reduce the chance of brain edema and protect the brain tissue by decreasing the expression of AQP-4.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2010年第4期366-369,共4页
Chinese Journal of Neuromedicine
