摘要
采用分子生物学方法构建16SrDNA基因文库,研究鸡粪沼气池发酵液中产甲烷菌的菌群结构。随机分析文库中50个克隆的16SrDNA基因序列,结果发现,其中46个克隆属于产甲烷菌属,与Methanogenium marinum strain AK-1菌株的相似性为99%-100%,占总数的92%;3个克隆(KD525、KD526和KD567)属于甲烷袋状菌属,与Methanoculleus sp.dm2菌株的相似性均为99%,占总数的6%;1个克隆(KD519)属于甲烷粒菌属,与Methanocorpusculum bavaricum菌株的相似性为99%,占总数的2%。另外,同样分析了样品中甲基辅酶M还原酶alpha亚基mcrA基因氨基酸序列的差异。气相色谱法分析结果显示,发酵液中甲酸含量为28.85g/L,约占总有机酸含量的81.7%;沼气的主要成分为甲烷、二氧化碳和氢气,分别约占总气体量的55.5%、41.1%和3.2%。
Methanogenic diversity was characterized in an anaerobic digester with chicken feces by using a culture-independent approach. 50 clones of a 16S rDNA gene library were randomly selected and their sequences were phylogenetically analyzed. 46 of them （92%） was determined belonging to Methanogenium with similarities to Methanogenium marinum strain AK-1 between 99% to 100%; 3 clones （6%）, KD525, KD526, and KD567, belonging to Methanoculleus with similarities 99% to Methanoculleus sp. dm2; one clone （2%）, KD519, belonging to Methanocorpusculum with a similarity 99% to Methanocorpusculum bavaricum. Further more, the phylogenetic analyse of alpha subunit A of methyl-coenzyme M reductase （mcrA） clones were also performed. Gas chromatography assay showed that the content of formic acid was 28.85 g/L, 81.7% of the total volatile fatty acid of the fresh fermentation slurry; and the produced biogas from the digester was also determined, mainly composing of methane （55.5%）, carbon dioxide （41.1%）,and hydrogen （3.2%）.
出处
《微生物学通报》
CAS
CSCD
北大核心
2010年第4期508-512,共5页
Microbiology China
基金
天津科技大学启动基金项目(No.0200135)
关键词
鸡粪沼气池
产甲烷菌
多样性
Anaerobic digester with chicken feces, Methanogen, Diversity
