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一株高效纤维素降解菌株的分离鉴定及其酶学性质 被引量:52

Isolation and Identification of a Cellulose Degrading Bacterium and Its Cellulase Characterization
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摘要 从黄浦江淀山湖的水底沉积物中筛选分离得到一株产纤维素酶的菌株。经细菌形态观察,生理生化实验并结合16SrRNA序列分析,鉴定该菌为蜡状芽孢杆菌(Bacillus cereus),同时发现其在系统发育树中处于一个独立的分支,推测该菌为Bacillus属中一个新的亚种。对该菌株产酶及酶活特性进行了初步研究,发现纤维素酶的产生与细菌的生长密切相关。该菌株在37°C,pH7.0的条件下,发酵66h后纤维素酶活达到最高值4.58U/mL。 A cellulose degrading strain DSH was isolated from the sediment of Dianshan Lake, Huangpu River, Shanghai. Basing on morphology, biochemical and physiological characterization, DSH was characterized as Bacillus cereus. A phylogenetic tree was constructed with the published 16S rRNA sequences of relative bacteria species. In the phylogenetic tree, the DSH strain is in a new branch which has the closest relative to Bacillus cereus strain JBE0004 with 99.78% sequence similarity. The generation of cellulose was closely correlated to its growth rate. The highest cellulose activity of DSH strain was 4.58 U/mL when grown at 37°C, pH 7.0 for 66 h.
出处 《微生物学通报》 CAS CSCD 北大核心 2010年第4期524-528,共5页 Microbiology China
基金 全国优秀博士学位论文作者专项资金资助项目(No.200549) 国家自然科学基金项目(No.50874032) 上海市重点学科建设项目(No.B604)
关键词 纤维素酶 降解 16S RRNA 蜡状芽孢杆菌 Cellulose, Degrading, 16S rRNA, Bacillus cereus
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参考文献14

  • 1Zhang YHP, Himmel ME, Mielenz JR. Outlook for cellulose improvement: screening and selection strategies. Biotechnology Advances, 2006(24): 452-481.
  • 2Lyndl R, Paul JW, Van ZYL WH, et al. Microbial cellulose utilization: fundamentals and biotechnology. Microbiology and Molecular Biology Reviews, 2002, 66(3): 506-577.
  • 3Hill J, Nelson E, Tilman D, et al. Environmental, economic and energetic costs and benefits of biodiesel and ethanol biofuels. Proc Natl Acad Sci USA, 2006, 103(30): 11206-11210.
  • 4Zeng X, Ma Y, Ma L. Utilization of straw in biomass energy in China. Renewable and Sustainable Energy Reviews, 2007(11): 976-987.
  • 5陈冠军,杜宗军,高培基.耐碱性真菌纤维素酶生产菌的筛选及酶学性质的初步研究[J].工业微生物,2000,30(4):23-27. 被引量:24
  • 6刘森林.微生物学实验创新教学体系的研究与实践[J].微生物学通报,2005,32(4):153-155. 被引量:77
  • 7Lane DJ. 16S/23S rRNA sequencing. STACKEBRANDT E, GOODFELLOW M, eds. Nucleic Acid Techniques in bacterial Systematics, 1991 : 115-175.
  • 8Thompson JD, Gibson TJ, Plewniak F, et al. The Clustal X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 1997(4): 4876-4882.
  • 9Kumar S, Tamura K, Nei M. MEGA3: Integrated software or molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics, 2004(5): 150-163.
  • 10Hardin MT, Mitchell DA, Howes T. Approach to designing rotating drum bioreactors for solid state fermentation on the basis of dimensionless design factors. Biotechnology and Bioengineering, 2000, 67(3): 274-282.

二级参考文献19

  • 1高培基 等.纤维素微生物及纤维素酶技术[M].,1994.3-4.
  • 2曲音波 高丧事基 等.斜卧青霉纤维素酶的研究Ⅰ JU1菌株的产酶及酶作用条件[J].山东大学学报,1986,21(1):140-143.
  • 3那安.-[J].微生物学报,1982,22(4):333-338.
  • 4曲音波.[D].山东大学,1986.
  • 5无锡轻院等八院校编.发酵工艺学[M].,1984.238- 243.
  • 6李佐虎.第五届全国生化工程学术会论文集[M].北京:化学工业出版社,1993.12- 17.
  • 7高培基,纤维素微生物及纤维素酶技术,1994年,3页
  • 8Yu B,J Chin Agric Chem Soc,1993年,31卷,5期,614页
  • 9Tong C C,rtanika,1992年,15卷,1期,45页
  • 10白毓谦,微生物实验技术,1987年,459页

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