摘要
本文对枯草芽孢杆菌在不同碳源、氮源培养基的生长及破乳效能进行了研究,并通过正交试验对枯草芽孢杆菌的发酵条件进行优化结果表明,单一碳源葡萄糖和混合碳源葡萄糖+液体石蜡的培养基可提高枯草芽孢杆菌的发酵产量;单一碳源葡萄糖、混合碳源葡萄糖+汽油和以硝酸铵+酵母膏为氮源的菌液有较高的破乳效能;在正交试验中,培养温度对枯草芽孢杆菌的发酵产量影响最大,其最优组合为:培养温度25°C,摇床转数140r/min,培养pH值7.0,接菌量6mL,培养时间24h。摇床转数对枯草芽孢杆菌的发酵产物的破乳效能影响最大,优化结果为:培养温度25°C,摇床转数140r/min,pH值7.0,培养时间20h。
Growth and de-emulsification efficiency of Bacillus subtilis under different carbon and nitrogen sources were investigated. The fermentation conditions were also optimized with orthogonal tests. The results showed that medium containing glucose as sole carbon source and compound medium containing glucose and liquid paraffin as carbon sources could enhance the fermentation production of the Bacillus subtilis. Higher de-emulsification efficiency was obtain with medium utilized glucose as sole carbon source, compound medium utilizes glucose and gasoline as carbon source as well as medium utilized ammonium nitrate and yeast extract as nitrogen sources. The orthogonal tests showed that the incubation temperature was the most influential factor for the fermentation production of the Bacillus subtilis. The optimal combination for fementation was as follows： incubation temperature 25°C, shaker revolution 140 r/min, pH 7.0, inoculum concentration 6 mL and incubation time 24 h. The most influential factor for the de-emulsification efficiency of the fermentation products of the Bacillus subtilis＇s fermentation products was the shaker revolution. The optimal combination for demulsification efficiency was as follows： incubation temperature 25°C, shaker revolution 140 r/min, pH 7.0 and incubation time 24 h.
出处
《微生物学通报》
CAS
CSCD
北大核心
2010年第4期580-585,共6页
Microbiology China
基金
国家863计划项目(No.2009AA062906)
黑龙江省普通高等学校青年学术骨干支持计划项目(No.1154G31)
博士后研究人员落户黑龙江科研启动金资助项目(2009)
关键词
枯草芽孢杆菌
生物破乳剂
发酵条件
破乳效能
Bacillus subtilis, Biological de-emulsifier, Fermentation conditions, De-emulsification activity
