摘要
目的观察经皮自体骨髓移植在骨缺损瘢痕组织内的成骨作用。方法选健康家兔１８只，建立双侧桡骨中段骨及骨膜缺损１ｃｍ模型，两骨断端髓腔用骨蜡封闭。６周后，实验侧（右）桡骨骨缺损区经皮注射自体红骨髓２ｍｌ；对照侧（左）桡骨骨缺损区注射自身外周血２ｍｌ。于注射前及注射后不同时间内分别行Ｘ线、组织学检查及骨缺损区瘢痕组织内钙磷含量的测定。结果注射后１周，实验侧Ｘ线显示以骨缺损为中心形成一个密度增高的弧形软组织影，组织切片为大量新生的软骨组织；对照侧Ｘ线片示骨缺损区无改变，组织切片为纤维结缔组织。注射后４周，实验侧Ｘ线显示骨缺损区新骨形成逐渐增多，骨缺损间隙变小，平均１．５ｍｍ，组织切片见骨小梁增多、变宽；对照侧Ｘ线示骨缺损间隙平均８ｍｍ，组织切片见骨缺损区为纤维结缔组织。注射后１２周，实验侧Ｘ线示骨缺损间隙平均１ｍｍ，组织切片为逐渐成熟的骨组织；对照侧骨缺损间隙平均７．５ｍｍ，两骨端萎缩，呈“盲肠末端”改变，组织学检查为致密的结缔组织。骨缺损区瘢痕组织的钙、磷含量，实验侧均高于对照侧。结论经皮自体骨髓注射在骨缺损区的瘢痕组织内有明显成骨作用，外周血注射不能成骨。
Objective To observe osteogenesis of percutaneous autogenous bone marrow into cicatrix of bone defect.Methods 18 rabbits were used to observe the osteogenesis of percutaneous autogenous bone marrow into cicatrix of bone defect.A 1cm bone defect model was made in each side of the radius.And 2ml autogenous bone marrow was injected in the right radial bone defect after 6 weeks,and 2ml peripheral blood in the left side.The animals were scarificed in 1,2,3,4,12 weeks after injection.Bone formation was studied with histologic technique,X ray examination and Ca P quantitative estimates.Results After 1 week of injection,a big shadow of softtissue was found around the bone defect in the right side(bone marrow).Histologically a lot of new cartilage like tissues were found.In the left side(peripheral blood), no change was seen.After 4 weeks of injection, many of new bone like shadows were observed around the defect by X ray examination in the right side.The defect was smaller(average 1.5mm) and bone formation was found.But in the left side,no signs for osteogenesis were noted.In 12 weeks defect was smaller(average 1mm).Histologic sections proved well developed bone tissue.In the left side,no bone formation found.Conclusion Autogenous bone marrow can induce osteogenesis in cicatric bone defect, whereas no osteogenesis is found in the peripheral blood injection side.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1998年第2期74-76,I013,共4页
Chinese Journal of Surgery
关键词
骨髓移植
成骨细胞
瘢痕
动物实验
自体移植
Bone marrow transplantation Osteoblasts Cicatrix Animals,laboratory
