摘要
目的使放射免疫显像诊断膀胱癌更加简便、安全、可靠。方法采用抗人膀胱癌单克隆抗体ＢＤＩ－１通过２－巯基乙醇直接还原法标记９９ｍＴｃ（９９ｍＴｃ）制备了９９ｍＴｃ－ＢＤＩ－１。结果标记率为６９．９％，放化纯度大于９５％。３３例患者接受了通过膀胱内灌注的放射免疫显像诊断，其中４例非膀胱癌患者均未见显像，２９例膀胱癌患者肿瘤显像阳性率为８８．５％。原发和复发肿瘤均可获得清晰的显像，显像肿瘤最小为０．５ｃｍ，肿瘤细胞分级包括Ｇ１～Ｇ３。显像结果与膀胱镜检结果完全符合者占６５．２％，１１例多发肿瘤中有７例（６３．６％）显像结果在肿瘤数目、位置及大小上与膀胱镜检有所差异。结论该方法为膀胱癌的诊断提供了一种新的安全有效的手段。
Objective To make radioimmunoimaging of bladder tumor simple,safe and reliable.Methods Anti human bladder carcinoma cell line BIU 87 monoclonal antibody BDI 1 was labelled with technetium 99m by direct mercaptoethanol reducing method.Results The labelling rate was 69 6% and the radiochemistry pruity was higher than 95%. 33 patients received radioimmunoimaging of bladder tumor with the intravesical administration of technetium 99m labelled monoclonal antibody.The false positive rate was zero.88 5% of all 29 bladder cancer patients had got positive immunoscintigraphy results, and 65 2% of them were confirmed by cystoscopic results.Both primary and recurrent tumor could be detected clearly and the smallest tumor detected was 0 5cm in diameter.There were some differences between the radioimmunoimaging and cystoscopic results in 7 of 11 multitumor cases,which included tumor size,number and location.Conclusion It is required to use more suitable isotopes and/or monoclonal antibodies to increase its sensitivity.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1998年第1期54-56,I005,共4页
Chinese Journal of Surgery
基金
国家教委博士点基金
