期刊文献+

Norplant皮下埋植后子宫内膜形态和雌、孕激素受体含量的变化 被引量:10

The Changes of Sex Steroid Receptors and Morphology in Norplant Treated Endometrium
原文传递
导出
摘要 目的探讨Norplant皮下埋植后子宫内膜形态和雌、孕激素受体(ER、PR)含量的变化与子宫异常出血的关系。方法应用免疫组化、WesternBlot和原位杂交技术结合计算机图像分析,观察16例Norplant皮下埋植后和23例正常周期的内膜的形态学改变、细胞增殖状态及ER、PR蛋白及其mRNA的表达。结果埋植Norplant后,内膜DNA含量降低;螺旋动脉数量减少,腺体的构成比例降低;腺体构成比例与雌二醇(E2)水平呈正相关,与子宫异常出血呈负相关,与年龄和体重无相关性,随使用期延长,腺体构成比增加。埋植后,内膜ER含量低于月经周期各期水平,但PR含量相当于或高于月经周期最高水平。ER、PRmRNA表达减弱,以ERmRNA表达降低更明显。结论内膜腺体和螺旋动脉再生修复不良和ER、PR表达异常。 Objective To study the relationship between the changes of sex steroid receptors as well as endometrial morphology and abnormal uterine bleeding induced by Norplant. Methods Immunohistochemistry, Western Blot, in situ hybridization and computer image analysis were employed to demonstrate the expression of estrone receptor(ER)、progesterone receptor(PR) protein and mRNA as well as morphometric analysis of the endometrium in Norplant users. Results The content of DNA and the numbers of spiral arterioles decreased in Norplant treated endometrium. The percentage of glandular area in Norplant endometrium was much lower than that in cyclic endometrium. A statistically significant correlation was observed between the percentage of area of glands and abnormal uterine bleeding, as well as serum estrogen(E2) level, but E2 level had no direct relationship with the bleeding abnormality. As the time growing, the percentage of glandular area increased. Immunostaining and relative concentration of glandular and stromal ER in Norplant treated endometrium were significantly weaker and less than all the stage of cyclic endometrium. However, PR, especially stromal PR immunostaining was very intense, which was the same or more than that in all the stage of cyclic endometrium. In situ hybridization detected that the ER and PR mRNA was lower in Norplant treated endometrium than that in cyclic endometrium. The ER mRNA expression was more less abundant than PR mRNA expression. Conclusion These results suggested that poor repair and regeneration of endometrium(especially glands and spiral arterioles) and abnormal expression of ER and PR may be related to pathogenesis of abnormal uterine bleeding induced by Norplant implant.
出处 《中华妇产科杂志》 CAS CSCD 北大核心 1998年第8期493-495,I014,共3页 Chinese Journal of Obstetrics and Gynecology
关键词 子宫内膜 性激素 受体 子宫出血 NORPLANT Norplant Endometrium Receptors progesterone Receptors estrogen Uterine hemorrhage
  • 相关文献

参考文献3

  • 1金冬雁(译),分子克隆实验指南(第2版),1996年,16,870页
  • 2夏雅仙,国外医学.计划生育分册,1995年,14卷,14页
  • 3何美丽,国外医学.计划生育分册,1991年,11卷,196页

同被引文献57

引证文献10

二级引证文献21

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部