摘要
建立了检测用ｖｅｒｏ细胞增殖ＩＢＤＶＸ、Ｎ毒滴度的抗原捕捉间接ＥＬＩＳＡ法（ＡＣＩ－ＥＬＩＳＡ），并与常规ＴＣＩＤ５０测定法作了比较。结果表明，ＡＣＩ－ＥＬＩＳＡ法测定的Ｐ／Ｎ值与ＴＣＩＤ５０呈明显的正相关，与Ｘ、Ｎ毒的相关系数分别为０．９６６８（Ｐ＜０．００１），０．９３８（Ｐ＜０．００１）。ＡＣＩ－ＥＬＩＳＡ法可用于ＩＢＤＶ－ｖｅｒｏ细胞毒的定量监测。
An antigen capture indirect ELISA(ACI ELISA)for detecting viral titres in vero cells is developed.ACI ELISA and TCID50 are used for detecting X strain and N strain of IBDV in vero cell supernatant, the result showed that P/N values of ACI ELISA and TCID50 in vero cells had a good positive correlation (P<0.001).The correlation coefficient of ACI ELISA to X and N strains are 0.966 8,0.938, respectively.ACI ELISA can be used for detection of IBDV in vero cells.
出处
《天津农业科学》
CAS
1998年第A00期12-15,共4页
Tianjin Agricultural Sciences
