摘要
目的通过观察钒化钠对人胃癌SGC7901细胞周期蛋白cyclinD1表达的影响,探讨其对人胃癌SGC7901细胞增殖抑制的作用机理。方法应用MTT法测定钒化钠对人胃癌SGC7901细胞的生长抑制作用;用Western-blot法检测钒化钠对人胃癌SGC7901细胞周期蛋白cyclinD1表达水平的变化。结果一定浓度的钒化钠使人胃癌SGC7901细胞增殖周期密切相关的cyclinD1蛋白表达降低。结论一定浓度的钒化钠能明显抑制人胃癌SGC7901细胞的生长,钒化钠的抗肿瘤机制可能与cyclinD1蛋白表达降低有关。
Objective To investigate the effect of sodium vanadate on cyclinD1 expression in human gastric cancer cell line SGC7901 and explore its potential mechanisms of inhibiting proliferation. Methods Cell growth inhibition in vitro was evaluated with MTr assay in human gastric cancer cell line SGC7901. Western blot analysis was applied to determine modification of cyclinD1 proteins of human gastric cancer cell line SGC7901. Results The expression of CyclinD1 was evidently decreased by sodium vanadate of certain concentration. Conclusion Sodium vanadate inhibits the proliferation of human gastric cancer cell line SGC7901 significantly, the antitumor mechanism of sodium vanadate may be related to down-regulation of CyclinD1 expression.
出处
《中国临床新医学》
2010年第4期315-318,共4页
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
